Faik P, Morgan M, Naftalin R J, Rist R J
Department of Biochemistry, United Medical School of Guy's, St. Thomas' Hospital London, U.K.
Biochem J. 1989 May 15;260(1):153-5. doi: 10.1042/bj2600153.
Hexokinase-deficient mutants and wild-type Chinese-hamster ovary cells have been used to investigate the role of hexokinase in uptake and accumulation of 2-D-deoxyglucose (2-dGlc). The evidence for a specific sugar transport system in both types of cells is that there is similar saturable phloretin-sensitive uptake of 2-dGlc and 3-O-methyl-D-glucose (3-OMG) in both types of cell. In wild-type cells, 2-dGlc is accumulated to a tissue:medium ratio of 10- and in the mutant only 3-fold; 3-OMG is not accumulated by either mutant or wild-type cells. The evidence that hexokinase affects the membrane transport process is that the rate of exit of free 2-dGlc from wild-type cells is 5-fold less than from mutant cells, whereas there is no difference in the rate of loss of 3-OMG between mutant and wild-type cells.
己糖激酶缺陷型突变体和野生型中国仓鼠卵巢细胞已被用于研究己糖激酶在2-脱氧葡萄糖（2-dGlc）摄取和积累中的作用。两种细胞类型中存在特定糖转运系统的证据是，两种细胞类型中2-dGlc和3-O-甲基-D-葡萄糖（3-OMG）的摄取都有类似的可饱和的根皮素敏感性。在野生型细胞中，2-dGlc积累至组织与培养基的比例为10倍，而在突变体中仅为3倍；突变体和野生型细胞均不积累3-OMG。己糖激酶影响膜转运过程的证据是，游离2-dGlc从野生型细胞中流出的速率比从突变体细胞中流出的速率低5倍，而突变体和野生型细胞之间3-OMG的损失速率没有差异。
