2-脱氧-D-葡萄糖在野生型和己糖激酶缺陷型中国仓鼠卵巢（CHO）培养细胞中的转运与积累
Transport and accumulation of 2-deoxy-D-glucose in wild-type and hexokinase-deficient cultured Chinese-hamster ovary (CHO) cells.
作者信息
Faik P, Morgan M, Naftalin R J, Rist R J
机构信息
Department of Biochemistry, United Medical School of Guy's, St. Thomas' Hospital London, U.K.
出版信息
Biochem J. 1989 May 15;260(1):153-5. doi: 10.1042/bj2600153.
Abstract
Hexokinase-deficient mutants and wild-type Chinese-hamster ovary cells have been used to investigate the role of hexokinase in uptake and accumulation of 2-D-deoxyglucose (2-dGlc). The evidence for a specific sugar transport system in both types of cells is that there is similar saturable phloretin-sensitive uptake of 2-dGlc and 3-O-methyl-D-glucose (3-OMG) in both types of cell. In wild-type cells, 2-dGlc is accumulated to a tissue:medium ratio of 10- and in the mutant only 3-fold; 3-OMG is not accumulated by either mutant or wild-type cells. The evidence that hexokinase affects the membrane transport process is that the rate of exit of free 2-dGlc from wild-type cells is 5-fold less than from mutant cells, whereas there is no difference in the rate of loss of 3-OMG between mutant and wild-type cells.
摘要
己糖激酶缺陷型突变体和野生型中国仓鼠卵巢细胞已被用于研究己糖激酶在2-脱氧葡萄糖（2-dGlc）摄取和积累中的作用。两种细胞类型中存在特定糖转运系统的证据是，两种细胞类型中2-dGlc和3-O-甲基-D-葡萄糖（3-OMG）的摄取都有类似的可饱和的根皮素敏感性。在野生型细胞中，2-dGlc积累至组织与培养基的比例为10倍，而在突变体中仅为3倍；突变体和野生型细胞均不积累3-OMG。己糖激酶影响膜转运过程的证据是，游离2-dGlc从野生型细胞中流出的速率比从突变体细胞中流出的速率低5倍，而突变体和野生型细胞之间3-OMG的损失速率没有差异。
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本文引用的文献
1
Studies on the mechanism of intestinal absorption of sugars. VI. The specificity and other properties of Na ion-dependent entrance of sugars into intestinal tissue under anaerobic conditions, in vitro.糖类肠道吸收机制的研究。VI. 体外厌氧条件下糖类依赖钠离子进入肠道组织的特异性及其他特性。
Biochim Biophys Acta. 1962 May 7;59:94-102. doi: 10.1016/0006-3002(62)90700-x.
2
The regulation of carbohydrate metabolism in animal cells: isolation of a glycolytic variant of Chinese hamster ovary cells.动物细胞中碳水化合物代谢的调节：中国仓鼠卵巢细胞糖酵解变体的分离
Cell Biol Int Rep. 1980 Feb;4(2):121-7. doi: 10.1016/0309-1651(80)90066-1.
3
Down-regulation of the hexose transport system: metabolic basis studied with a fibroblast mutant lacking phosphoglucose isomerase.己糖转运系统的下调：利用缺乏磷酸葡萄糖异构酶的成纤维细胞突变体进行的代谢基础研究
Proc Natl Acad Sci U S A. 1982 Jun;79(12):3777-9. doi: 10.1073/pnas.79.12.3777.
4
Isolation of a Chinese hamster fibroblast mutant defective in hexose transport and aerobic glycolysis: its use to dissect the malignant phenotype.分离出一种在己糖转运和有氧糖酵解方面存在缺陷的中国仓鼠成纤维细胞突变体：利用其剖析恶性表型。
Proc Natl Acad Sci U S A. 1980 May;77(5):2698-701. doi: 10.1073/pnas.77.5.2698.
5
Carbohydrate metabolism in cultured animal cells.
Biosci Rep. 1981 Sep;1(9):669-86. doi: 10.1007/BF01116465.
6
Induction and isolation of auxotrophic mutants in mammalian cells.哺乳动物细胞中营养缺陷型突变体的诱导与分离
Methods Cell Biol. 1974;8(0):23-39. doi: 10.1016/s0091-679x(08)60441-0.
7
8
A model for accelerated uptake and accumulation of sugars arising from phosphorylation at the inner surface of the cell membrane.一种由细胞膜内表面磷酸化引发的糖快速摄取和积累模型。
Biochim Biophys Acta. 1987 Feb 12;897(1):93-111. doi: 10.1016/0005-2736(87)90318-x.
9
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10
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
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