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多糖生物合成相关基因解释了中国太湖梅梁湾微囊藻群体的表型-基因型相关性。

Polysaccharide biosynthesis-related genes explain phenotype-genotype correlation of Microcystis colonies in Meiliang Bay of Lake Taihu, China.

作者信息

Xu Shutu, Sun Qianqian, Zhou Xiaohua, Tan Xiao, Xiao Man, Zhu Wei, Li Ming

机构信息

College of Agronomy, Northwest A &F University, Yangling 712100, PR China.

College of Resources and Environment, Northwest A &F University, Yangling 712100, PR China.

出版信息

Sci Rep. 2016 Oct 18;6:35551. doi: 10.1038/srep35551.

DOI:10.1038/srep35551
PMID:27752091
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5067575/
Abstract

The 16S rDNA, 16S-23S rDNA-ITS, cpcBA-IGS, mcy gene and several polysaccharide biosynthesis-related genes (epsL and TagH) were analyzed along with the identification of the morphology of Microcystis colonies collected in Lake Taihu in 2014. M. wesenbergii colonies could be distinguished directly from other colonies using espL. TagH divided all of the samples into two clusters but failed to distinguish different phenotypes. Our results indicated that neither morphology nor molecular tools including 16S rDNA, 16S-23S ITS and cpcBA-IGS could distinguish toxic and non-toxic species among the identified Microcystis species. No obvious relationship was detected between the phenotypes of Microcystis and their genotypes using 16S, 16S-23S and cpcBA-IGS, but polysaccharide biosynthesis-related genes may distinguish the Microcystis phenotypes. Furthermore, the sequences of the polysaccharide biosynthesis-related genes (espL and TagH) extracted from Microcystis scums collected throughout 2015 was analyzed. Samples dominated by M. ichthyoblabe (60-100%) and M. wesenbergii (60-100%) were divided into different clade by both espL and TagH, respectively. Therefore, it was confirmed that M. wesenbergii and M. ichthyoblabe could be distinguished by the polysaccharide biosynthesis-related genes (espL and TagH). This study is of great significance in filling the gap between classification of molecular biology and the morphological taxonomy of Microcystis.

摘要

对2014年在太湖采集的微囊藻群体的形态进行鉴定的同时，分析了16S rDNA、16S - 23S rDNA - ITS、cpcBA - IGS、mcy基因以及几个与多糖生物合成相关的基因（epsL和TagH）。利用espL可直接将惠氏微囊藻群体与其他群体区分开来。TagH将所有样本分为两个聚类，但无法区分不同的表型。我们的结果表明，无论是形态学还是包括16S rDNA、16S - 23S ITS和cpcBA - IGS在内的分子工具，都无法在已鉴定的微囊藻物种中区分有毒和无毒物种。使用16S、16S - 23S和cpcBA - IGS未检测到微囊藻表型与其基因型之间存在明显关系，但与多糖生物合成相关的基因可能区分微囊藻表型。此外，还分析了2015年全年从微囊藻浮沫中提取的与多糖生物合成相关基因（epsL和TagH）的序列。以鱼害微囊藻（60 - 100%）和惠氏微囊藻（60 - 100%）为主的样本分别被espL和TagH分为不同的进化枝。因此，证实了惠氏微囊藻和鱼害微囊藻可通过与多糖生物合成相关的基因（epsL和TagH）区分开来。本研究对于填补微囊藻分子生物学分类与形态分类学之间的空白具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/71c3e82c124a/srep35551-f8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/71c3e82c124a/srep35551-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/779aed546af6/srep35551-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/b59a4c506be6/srep35551-f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/50ef0613db54/srep35551-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/96b0f760f668/srep35551-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/d5c15083c382/srep35551-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46f5/5067575/71c3e82c124a/srep35551-f8.jpg

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本文引用的文献

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