大鼠肝脏的胞质谷胱甘肽转移酶。α类谷胱甘肽转移酶8-8的一级结构及低丰度μ类谷胱甘肽转移酶的特性
Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases.
作者信息
Alin P, Jensson H, Cederlund E, Jörnvall H, Mannervik B
机构信息
Department of Biochemistry, University of Stockholm, Sweden.
出版信息
Biochem J. 1989 Jul 15;261(2):531-9. doi: 10.1042/bj2610531.
Abstract
Six GSH transferases with neutral/acidic isoelectric points were purified from the cytosol fraction of rat liver. Four transferases are class Mu enzymes related to the previously characterized GSH transferases 3-3, 4-4 and 6-6, as judged by structural and enzymic properties. Two additional GSH transferases are distinguished by high specific activities with 4-hydroxyalk-2-enals, toxic products of lipid peroxidation. The most abundant of these two enzymes, GSH transferase 8-8, a class Alpha enzyme, has earlier been identified in rat lung and kidney. The amino acid sequence of subunit 8 was determined and showed a typical class Alpha GSH transferase structure including an N-acetylated N-terminal methionine residue.
摘要
从大鼠肝脏的胞质溶胶部分纯化出六种具有中性/酸性等电点的谷胱甘肽转移酶。根据结构和酶学特性判断，其中四种转移酶是与先前鉴定的谷胱甘肽转移酶3-3、4-4和6-6相关的Mu类酶。另外两种谷胱甘肽转移酶的特点是对脂质过氧化的有毒产物4-羟基链-2-烯醛具有高比活性。这两种酶中含量最丰富的谷胱甘肽转移酶8-8是一种Alpha类酶，此前已在大鼠肺和肾中鉴定出来。确定了亚基8的氨基酸序列，其显示出典型的Alpha类谷胱甘肽转移酶结构，包括一个N-乙酰化的N端甲硫氨酸残基。
相似文献
1
Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases.
Biochem J. 1989 Jul 15;261(2):531-9. doi: 10.1042/bj2610531.
2
Cytosolic rat liver glutathione transferase 4-4. Primary structure of the protein reveals extensive differences between homologous glutathione transferases of classes alpha and mu.
Eur J Biochem. 1986 Apr 15;156(2):343-50. doi: 10.1111/j.1432-1033.1986.tb09588.x.
3
Purification and characterization of a new cytosolic glutathione S-transferase (glutathione S-transferase X) from rat liver.
Biochem J. 1983 Dec 1;215(3):617-25. doi: 10.1042/bj2150617.
4
Purification of cytosolic glutathione transferases from Schistocephalus solidus (plerocercoid): interaction with anthelmintics and products of lipid peroxidation.
Mol Biochem Parasitol. 1989 Sep;36(2):187-96. doi: 10.1016/0166-6851(89)90191-6.
5
Purification and characterization of hepatic glutathione S-transferases of rhesus monkeys. A family of enzymes similar to the human hepatic glutathione S-transferases.
Biochem J. 1988 Apr 1;251(1):81-8. doi: 10.1042/bj2510081.
6
Isoelectric focusing of glutathione S-transferases from rat liver and kidney.
Biochem J. 1978 Dec 1;175(3):937-43. doi: 10.1042/bj1750937.
7
Propylthiouracil inducible glutathione transferases. Selective induction of ligandin (glutathione transferase 1-1).
Biochem Pharmacol. 1986 Jun 1;35(11):1835-9. doi: 10.1016/0006-2952(86)90300-x.
8
Guinea pig liver Mu-class glutathione S-transferase M1-2 cross-reacts with antibodies to both rat Mu- and theta-class glutathione S-transferases.
Arch Biochem Biophys. 1998 Jun 1;354(1):188-96. doi: 10.1006/abbi.1998.0649.
9
Detoxification of DNA hydroperoxide by glutathione transferases and the purification and characterization of glutathione transferases of the rat liver nucleus.
Biochem J. 1988 Sep 15;254(3):841-5. doi: 10.1042/bj2540841.
10
Novel theta class glutathione S-transferases Yrs-Yrs' and Yrs'-Yrs' in rat liver cytosol: their potent activity toward 5-sulfoxymethylchrysene, a reactive metabolite of the carcinogen 5-hydroxymethylchrysene.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):278-84. doi: 10.1006/bbrc.1994.1924.
引用本文的文献
1
Effect of Docosahexaenoic Acid Ingestion on Temporal Change in Urinary Excretion of Mercapturic Acid in ODS Rats.
J Clin Biochem Nutr. 2007 Nov;41(3):184-90. doi: 10.3164/jcbn.2007026.
2
Multiple isoforms of mitochondrial glutathione S-transferases and their differential induction under oxidative stress.
Biochem J. 2002 Aug 15;366(Pt 1):45-55. doi: 10.1042/BJ20020533.
3
(S)-preferential detoxification of 4-hydroxy-2(E)-nonenal enantiomers by hepatic glutathione S-transferase isoforms in guinea-pigs and rats.
Biochem J. 2001 Apr 1;355(Pt 1):237-44. doi: 10.1042/0264-6021:3550237.
4
4-Hydroxy-2(E)-nonenal enantiomers: (S)-selective inactivation of glyceraldehyde-3-phosphate dehydrogenase and detoxification by rat glutathione S-transferase A4-4.
Biochem J. 2000 Aug 1;349 Pt 3(Pt 3):729-35. doi: 10.1042/bj3490729.
5
Amino acid sequence of glutathione S-transferase rGSTM5* from rat testis.
Biochem J. 1998 Aug 1;333 ( Pt 3)(Pt 3):735-9. doi: 10.1042/bj3330735.
6
Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-alpha GST, rGSTA6*, in rat testis.
Biochem J. 1997 Apr 15;323 ( Pt 2)(Pt 2):503-10. doi: 10.1042/bj3230503.
7
Mass spectrometric analysis of rat liver cytosolic glutathione S-transferases: modifications are limited to N-terminal processing.
Biochem J. 1995 May 15;308 ( Pt 1)(Pt 1):69-75. doi: 10.1042/bj3080069.
8
Characterization of methylation of rat liver cytosolic glutathione S-transferases by using reverse-phase h.p.l.c. and chromatofocusing.
Biochem J. 1990 Sep 1;270(2):483-9. doi: 10.1042/bj2700483.
9
Rat spleen glutathione transferases. A new acidic form belonging to the Alpha class.
Biochem J. 1990 Mar 1;266(2):461-5. doi: 10.1042/bj2660461.
10
Structural distinction of rat GSH transferase subunit 10.
Biochem J. 1991 Mar 1;274 ( Pt 2)(Pt 2):619. doi: 10.1042/bj2740619a.
本文引用的文献
1
An enzyme from rat liver catalysing conjugations with glutathione.
Biochem J. 1961 Jun;79(3):516-24. doi: 10.1042/bj0790516.
2
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
3
Thioltransferase.
Methods Enzymol. 1981;77:281-5. doi: 10.1016/s0076-6879(81)77038-1.
4
Purification and characterization of three forms of glutathione S-transferase A. A comparative study of the major YaYa-, YbYb- and YcYc-containing glutathione S-transferases.
Biochem J. 1982 Dec 1;207(3):459-70. doi: 10.1042/bj2070459.
5
Evidence that the Ya and Yc subunits of glutathione transferase B (ligandin) are the products of separate genes.
Eur J Biochem. 1982 Sep 1;126(3):459-63. doi: 10.1111/j.1432-1033.1982.tb06802.x.
6
7
Bile acid inhibition of basic and neutral glutathione S-transferases in rat liver.
Biochem J. 1983 Dec 1;215(3):581-8. doi: 10.1042/bj2150581.
8
Glutathione transferases in rat testis.
Acta Chem Scand B. 1983;37(3):261-2.
9
Rat liver glutathione S-transferases. A study of the structure of the basic YbYb-containing enzymes.
Biochem J. 1983 Sep 1;213(3):625-33. doi: 10.1042/bj2130625.
10
Purification and characterization of glutathione S-transferases P, S and N. Isolation from rat liver of Yb1 Yn protein, the existence of which was predicted by subunit hybridization in vitro.
Biochem J. 1984 Dec 15;224(3):839-52. doi: 10.1042/bj2240839.
Zotero 插件