大鼠肝脏的胞质谷胱甘肽转移酶。α类谷胱甘肽转移酶8-8的一级结构及低丰度μ类谷胱甘肽转移酶的特性
Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases.
作者信息
Alin P, Jensson H, Cederlund E, Jörnvall H, Mannervik B
机构信息
Department of Biochemistry, University of Stockholm, Sweden.
出版信息
Biochem J. 1989 Jul 15;261(2):531-9. doi: 10.1042/bj2610531.
Abstract
Six GSH transferases with neutral/acidic isoelectric points were purified from the cytosol fraction of rat liver. Four transferases are class Mu enzymes related to the previously characterized GSH transferases 3-3, 4-4 and 6-6, as judged by structural and enzymic properties. Two additional GSH transferases are distinguished by high specific activities with 4-hydroxyalk-2-enals, toxic products of lipid peroxidation. The most abundant of these two enzymes, GSH transferase 8-8, a class Alpha enzyme, has earlier been identified in rat lung and kidney. The amino acid sequence of subunit 8 was determined and showed a typical class Alpha GSH transferase structure including an N-acetylated N-terminal methionine residue.
摘要
从大鼠肝脏的胞质溶胶部分纯化出六种具有中性/酸性等电点的谷胱甘肽转移酶。根据结构和酶学特性判断，其中四种转移酶是与先前鉴定的谷胱甘肽转移酶3-3、4-4和6-6相关的Mu类酶。另外两种谷胱甘肽转移酶的特点是对脂质过氧化的有毒产物4-羟基链-2-烯醛具有高比活性。这两种酶中含量最丰富的谷胱甘肽转移酶8-8是一种Alpha类酶，此前已在大鼠肺和肾中鉴定出来。确定了亚基8的氨基酸序列，其显示出典型的Alpha类谷胱甘肽转移酶结构，包括一个N-乙酰化的N端甲硫氨酸残基。
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