大鼠睾丸谷胱甘肽S-转移酶rGSTM5*的氨基酸序列。
Amino acid sequence of glutathione S-transferase rGSTM5* from rat testis.
作者信息
Tam M F, Hsieh C H, Tsai S P, Tam T C
机构信息
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Republic of China.
出版信息
Biochem J. 1998 Aug 1;333 ( Pt 3)(Pt 3):735-9. doi: 10.1042/bj3330735.
Abstract
Glutathione S-transferase rGSTM5* was isolated from rat testis with a combination of glutathione affinity column and reverse-phase column chromatography. The protein was digested with Achromobacter protease I or endoproteinase Arg-C. The peptide fragments were isolated for electrospray MS and N-terminal peptide sequencing analyses. The primary amino acid sequence of rGSTM5* comprises 217 residues and has a calculated average molecular mass of 25495.3 Da. The result is identical to that obtained for rGSTM5* with liquid chromatography-MS from a mixture of rat testicular GSTs. Therefore, rGSTM5* has not been post-translationally modified.
摘要
谷胱甘肽S-转移酶rGSTM5是通过谷胱甘肽亲和柱和反相柱色谱相结合的方法从大鼠睾丸中分离出来的。该蛋白质用无色杆菌蛋白酶I或内肽酶Arg-C进行消化。分离肽片段用于电喷雾质谱和N端肽测序分析。rGSTM5的一级氨基酸序列由217个残基组成，计算得出的平均分子量为25495.3道尔顿。该结果与从大鼠睾丸谷胱甘肽S-转移酶混合物中通过液相色谱-质谱法获得的rGSTM5结果一致。因此，rGSTM5未发生翻译后修饰。
相似文献
1
Amino acid sequence of glutathione S-transferase rGSTM5* from rat testis.大鼠睾丸谷胱甘肽S-转移酶rGSTM5*的氨基酸序列。
Biochem J. 1998 Aug 1;333 ( Pt 3)(Pt 3):735-9. doi: 10.1042/bj3330735.
2
Rationale for reclassification of a distinctive subdivision of mammalian class Mu glutathione S-transferases that are primarily expressed in testis.对主要在睾丸中表达的哺乳动物μ类谷胱甘肽S-转移酶一个独特亚类重新分类的理论依据。
J Biol Chem. 1998 Apr 17;273(16):9593-601. doi: 10.1074/jbc.273.16.9593.
3
Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-alpha GST, rGSTA6*, in rat testis.大鼠卵巢和睾丸胞质谷胱甘肽S-转移酶（GSTs）的质谱分析：在大鼠睾丸中鉴定出一种新型α类GST，即rGSTA6* 。
Biochem J. 1997 Apr 15;323 ( Pt 2)(Pt 2):503-10. doi: 10.1042/bj3230503.
4
Rat kidney glutathione S-transferase 1 subunits have C-terminal truncations.大鼠肾脏谷胱甘肽S-转移酶1亚基具有C端截短。
Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):1017-25. doi: 10.1042/bj3141017.
5
6
Mass spectrometric analysis of rat liver cytosolic glutathione S-transferases: modifications are limited to N-terminal processing.大鼠肝脏胞质谷胱甘肽S-转移酶的质谱分析：修饰仅限于N端加工。
Biochem J. 1995 May 15;308 ( Pt 1)(Pt 1):69-75. doi: 10.1042/bj3080069.
7
Characterization and cloning of avian-hepatic glutathione S-transferases.禽肝谷胱甘肽S-转移酶的特性鉴定与克隆
Biochem J. 1999 Oct 1;343 Pt 1(Pt 1):87-93.
8
Site-specific arylation of rat glutathione s-transferase A1 and A2 by bromobenzene metabolites in vivo.体内溴苯代谢产物对大鼠谷胱甘肽S-转移酶A1和A2的位点特异性芳基化作用。
Chem Res Toxicol. 2006 Nov;19(11):1426-34. doi: 10.1021/tx060142s.
9
Molecular identification of glutathione S-transferase gene and cDNAs of two isotypes from northern quahog (Mercenaria mercenaria).硬壳蛤（Mercenaria mercenaria）谷胱甘肽S-转移酶基因及两种同工型cDNA的分子鉴定
Comp Biochem Physiol B Biochem Mol Biol. 2009 Sep;154(1):25-36. doi: 10.1016/j.cbpb.2009.04.012. Epub 2009 May 3.
10
Tissue distribution of enzymic methylation of glutathione S-transferase and its effects on catalytic activity. Methylation of glutathione S-transferase 11-11 inhibits conjugating activity towards 1-chloro-2,4-dinitrobenzene.谷胱甘肽S-转移酶的酶促甲基化的组织分布及其对催化活性的影响。谷胱甘肽S-转移酶11-11的甲基化抑制其对1-氯-2,4-二硝基苯的结合活性。
Biochem J. 1992 Feb 15;282 ( Pt 1)(Pt 1):279-89. doi: 10.1042/bj2820279.
引用本文的文献
1
Glutathione and its related enzymes in the gonad of Nile Tilapia (Oreochromis niloticus).尼罗罗非鱼（尼罗口孵非鲫）性腺中的谷胱甘肽及其相关酶
Fish Physiol Biochem. 2016 Feb;42(1):353-64. doi: 10.1007/s10695-015-0143-9.
本文引用的文献
1
Characterization of chicken-liver glutathione S-transferase (GST) A1-1 and A2-2 isoenzymes and their site-directed mutants heterologously expressed in Escherichia coli: identification of Lys-15 and Ser-208 on cGSTA1-1 as residues interacting with ethacrynic acid.鸡肝谷胱甘肽S-转移酶（GST）A1-1和A2-2同工酶及其在大肠杆菌中异源表达的定点突变体的特性：鉴定cGSTA1-1上的Lys-15和Ser-208为与依他尼酸相互作用的残基。
Biochem J. 1997 Oct 15;327 ( Pt 2)(Pt 2):593-600. doi: 10.1042/bj3270593.
2
Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-alpha GST, rGSTA6*, in rat testis.大鼠卵巢和睾丸胞质谷胱甘肽S-转移酶（GSTs）的质谱分析：在大鼠睾丸中鉴定出一种新型α类GST，即rGSTA6* 。
Biochem J. 1997 Apr 15;323 ( Pt 2)(Pt 2):503-10. doi: 10.1042/bj3230503.
3
The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance.谷胱甘肽S-转移酶超基因家族：谷胱甘肽S-转移酶的调控及其同工酶在癌症化学保护和耐药性中的作用。
Crit Rev Biochem Mol Biol. 1995;30(6):445-600. doi: 10.3109/10409239509083491.
4
Rat kidney glutathione S-transferase 1 subunits have C-terminal truncations.大鼠肾脏谷胱甘肽S-转移酶1亚基具有C端截短。
Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):1017-25. doi: 10.1042/bj3141017.
5
Tyrosine 115 participates both in chemical and physical steps of the catalytic mechanism of a glutathione S-transferase.酪氨酸115参与了谷胱甘肽S-转移酶催化机制的化学和物理步骤。
J Biol Chem. 1993 Jun 5;268(16):11508-11.
6
Structure and function of the xenobiotic substrate binding site of a glutathione S-transferase as revealed by X-ray crystallographic analysis of product complexes with the diastereomers of 9-(S-glutathionyl)-10-hydroxy-9,10-dihydrophenanthrene.通过对9-(S-谷胱甘肽基)-10-羟基-9,10-二氢菲的非对映异构体产物复合物进行X射线晶体学分析揭示的谷胱甘肽S-转移酶的异源生物底物结合位点的结构与功能
Biochemistry. 1994 Feb 8;33(5):1043-52. doi: 10.1021/bi00171a002.
7
Rational reconstruction of the active site of a class mu glutathione S-transferase.
J Biol Chem. 1994 Dec 23;269(51):32373-9.
8
Mass spectrometric analysis of rat liver cytosolic glutathione S-transferases: modifications are limited to N-terminal processing.大鼠肝脏胞质谷胱甘肽S-转移酶的质谱分析：修饰仅限于N端加工。
Biochem J. 1995 May 15;308 ( Pt 1)(Pt 1):69-75. doi: 10.1042/bj3080069.
9
Purification of glutathione S-transferases by glutathione-affinity chromatography.通过谷胱甘肽亲和色谱法纯化谷胱甘肽S-转移酶。
Methods Enzymol. 1981;77:235-7. doi: 10.1016/s0076-6879(81)77031-9.
10
Thymine hydroperoxide, a substrate for rat Se-dependent glutathione peroxidase and glutathione transferase isoenzymes.胸腺嘧啶过氧化氢，大鼠硒依赖性谷胱甘肽过氧化物酶和谷胱甘肽转移酶同工酶的一种底物。
FEBS Lett. 1986 Oct 27;207(2):231-3. doi: 10.1016/0014-5793(86)81494-6.
Zotero 插件