Fairfoul Graham, McGuire Lynne I, Pal Suvankar, Ironside James W, Neumann Juliane, Christie Sharon, Joachim Catherine, Esiri Margaret, Evetts Samuel G, Rolinski Michal, Baig Fahd, Ruffmann Claudio, Wade-Martins Richard, Hu Michele T M, Parkkinen Laura, Green Alison J E
The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom.
The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom; Centre for Clinical Brain Sciences Western General Hospital Edinburgh EH4 2XU United Kingdom.
Ann Clin Transl Neurol. 2016 Aug 28;3(10):812-818. doi: 10.1002/acn3.338. eCollection 2016 Oct.
We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.
我们开发了一种基于新型实时震颤诱导转化RT-QuIC的检测方法，用于检测路易体痴呆和帕金森病患者大脑及脑脊液中的α-突触核蛋白聚集情况。该检测方法可分别以92%和95%的灵敏度检测出路易体痴呆和帕金森病脑脊液中的α-突触核蛋白聚集，与阿尔茨海默病及对照脑脊液相比，总体特异性为100%。经神经病理学证实为tau蛋白病（进行性核上性麻痹；皮质基底节变性）的患者检测结果为阴性。这些结果表明，脑脊液的RT-QuiC分析对于α-突触核蛋白病患者的早期临床评估可能具有重要价值。
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