Kitamura A, Nagasawa H, Kataoka H, Inoue T, Matsumoto S, Ando T, Suzuki A
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1989 Aug 30;163(1):520-6. doi: 10.1016/0006-291x(89)92168-2.
We have isolated two distinct pheromone-biosynthesis-activating neuropeptides (PBAN), named PBAN-I and -II, as fully oxidized forms of Met residues from adult heads of the silkworm, Bombyx mori. PBAN-I was identical with the PBAN which we had isolated before. The complete amino acid sequence of PBAN-I, a total of 33 amino acid residues, was determined as H-Leu-Ser-Glu-Asp-Met-Pro-Ala-Thr-Pro-Ala-Asp-Gln-Glu-Met-Tyr-Gln-Pro-As p-Pro- Glu-Glu-Met-Glu-Ser-Arg-Thr-Arg-Tyr-Phe-Ser-Pro-Arg-Leu-NH2. Synthetic PBAN-I after oxidation with H2O2 was chromatographically identical with the isolated PBAN-I. Examination of PBAN activity of synthetic analogues indicated that the carboxyl-terminal portion of PBAN-I was important for biological activity.
我们从家蚕成虫头部中分离出两种不同的信息素生物合成激活神经肽（PBAN），分别命名为PBAN-I和PBAN-II，它们是甲硫氨酸残基的完全氧化形式。PBAN-I与我们之前分离出的PBAN相同。确定了PBAN-I的完整氨基酸序列，共33个氨基酸残基，为H-亮氨酸-丝氨酸-谷氨酸-天冬氨酸-甲硫氨酸-脯氨酸-丙氨酸-苏氨酸-脯氨酸-丙氨酸-天冬氨酸-谷氨酰胺-谷氨酸-甲硫氨酸-酪氨酸-谷氨酰胺-脯氨酸-天冬氨酸-脯氨酸-谷氨酸-谷氨酸-甲硫氨酸-谷氨酸-丝氨酸-精氨酸-苏氨酸-精氨酸-酪氨酸-苯丙氨酸-丝氨酸-脯氨酸-精氨酸-亮氨酸-NH2。用过氧化氢氧化后的合成PBAN-I在色谱上与分离出的PBAN-I相同。对合成类似物的PBAN活性检测表明，PBAN-I的羧基末端部分对生物活性很重要。
