血浆中循环长非编码 HOX 转录反义基因间 RNA 作为乳腺癌诊断潜在生物标志物的研究

Circulating long non-coding HOX transcript antisense intergenic ribonucleic acid in plasma as a potential biomarker for diagnosis of breast cancer.

机构信息

Department of Laboratory Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, China.

Department of Laboratory Medicine, Sichuan Cancer Hospital, Chengdu, China.

出版信息

Thorac Cancer. 2016 Nov;7(6):627-632. doi: 10.1111/1759-7714.12373. Epub 2016 Aug 1.

DOI:10.1111/1759-7714.12373

PMID:27755794

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5093169/

Abstract

BACKGROUND

It is well known that HOX transcript antisense intergenic ribonucleic acid (HOTAIR) plays an important role in breast cancer (BC). However, whether circulating HOTAIR in plasma could be used for BC diagnosis and dynamic monitoring are unclear.

METHODS

We tested the expression levels of HOTAIR in 30 pairs of tissue samples and 148 plasma samples from BC patients by quantitative real time-polymerase chain reaction, and the correlation between plasma HOTAIR levels and clinical features were analyzed. Receiver operating characteristic curve (ROC) was used to assess the diagnostic power of plasma HOTAIR for BC. Furthermore, we explored the monitoring values of plasma HOTAIR for BC and analyzed the correlation of HOTAIR levels between plasma and corresponding tissues of the same patients.

RESULTS

The expression levels of HOTAIR were significantly higher in BC tissues and plasma than in the control ( P < 0.05). The expression levels of plasma HOTAIR were correlated with lymph node metastasis ( P = 0.018), estrogen receptor (ER) ( P = 0.012), c-erbB-2 (P = 0.006) and triple positive ( P = 0.015). The area under the ROC curve of plasma HOTAIR was 0.80 (sensitivity 69.2%; specificity 93.3%), which was higher than the carcinoembryonic antigen and carbohydrate antigen 15-3 values obtained. Moreover, plasma HOTAIR expression levels in postoperative patients were lower than those in preoperative patients ( P = 0.029) and were moderately correlated with the corresponding tissue levels of the same patients (r = 0.68, P < 0.0001).

CONCLUSION

These results indicated that HOTAIR may be a potential biomarker for the diagnosis of BC.

摘要

背景

HOX 转录反义基因间 RNA（HOTAIR）在乳腺癌（BC）中发挥着重要作用，这是众所周知的。然而，循环血浆中的 HOTAIR 是否可用于 BC 的诊断和动态监测尚不清楚。

方法

我们通过实时定量聚合酶链反应（qRT-PCR）检测了 30 对组织样本和 148 例 BC 患者的血浆样本中的 HOTAIR 表达水平，并分析了血浆 HOTAIR 水平与临床特征之间的相关性。使用受试者工作特征曲线（ROC）评估血浆 HOTAIR 对 BC 的诊断能力。此外，我们探讨了血浆 HOTAIR 对 BC 的监测价值，并分析了同一患者血浆和相应组织中 HOTAIR 水平的相关性。

结果

BC 组织和血浆中的 HOTAIR 表达水平明显高于对照组（P<0.05）。血浆 HOTAIR 的表达水平与淋巴结转移（P=0.018）、雌激素受体（ER）（P=0.012）、c-erbB-2（P=0.006）和三阳性（P=0.015）相关。血浆 HOTAIR 的 ROC 曲线下面积为 0.80（敏感性 69.2%；特异性 93.3%），高于癌胚抗原和糖链抗原 15-3 的值。此外，术后患者的血浆 HOTAIR 表达水平低于术前患者（P=0.029），并且与同一患者的相应组织水平中度相关（r=0.68，P<0.0001）。