Zhou Xiaoying, Yin Chengqiang, Dang Yini, Ye Feng, Zhang Guoxin
1] Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China [2] First Clinical Medical College of Nanjing Medical University, Nanjing 210029, China.
Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Sci Rep. 2015 Jun 22;5:11516. doi: 10.1038/srep11516.
Recent studies have demonstrated that long non-coding RNAs (lncRNAs) are regarded as useful tools for cancer detection, particularly for the early stage; however, little is known about their diagnostic impact on gastric cancer (GC). We hypothesized that GC-related lncRNAs might release into the circulation during tumor initiation and could be utilized to detect and monitor GC. 8 lncRNAs which previously found to be differently expressed in GC were selected as candidate targets for subsequent circulating lncRNA assay. After validating in 20 pairs of tissues and plasma in training set, H19 was selected for further analysis in another 70 patients and 70 controls. Plasma level of H19 was significantly higher in GC patients compared with normal controls (p < 0.0001). By receiver operating characteristic curve (ROC) analysis, the area under the ROC curve (AUC) was 0.838; p < 0.001; sensitivity, 82.9%; specificity, 72.9%). Furthermore, H19 expression enabled the differentiation of early stage GC from controls with AUC of 0.877; sensitivity, 85.5%; specificity, 80.1%. Besides, plasma levels of H19 were significantly lower in postoperative samples than preoperative samples (p = 0.001). In conclusion, plasma H19 could serve as a potential biomarker for diagnosis of GC, in particular for early tumor screening.
最近的研究表明，长链非编码RNA（lncRNAs）被视为癌症检测的有用工具，尤其是在早期阶段；然而，关于它们对胃癌（GC）的诊断影响却知之甚少。我们推测，与GC相关的lncRNAs可能在肿瘤发生过程中释放到循环系统中，并可用于检测和监测GC。选择先前发现在GC中差异表达的8种lncRNAs作为后续循环lncRNA检测的候选靶点。在训练集中对20对组织和血浆进行验证后，选择H19在另外70例患者和70例对照中进行进一步分析。与正常对照相比，GC患者血浆中H19水平显著更高（p < 0.0001）。通过受试者工作特征曲线（ROC）分析，ROC曲线下面积（AUC）为0.838；p < 0.001；敏感性为82.9%；特异性为72.9%。此外，H19表达能够区分早期GC与对照，AUC为0.877；敏感性为85.5%；特异性为80.1%。此外，术后样本中H19的血浆水平显著低于术前样本（p = 0.001）。总之，血浆H19可作为GC诊断的潜在生物标志物，特别是用于早期肿瘤筛查。
