Department of Microbiology, Panjab University, Chandigarh 160014, India.
Department of Biotechnology, D.A.V. College, Chandigarh 160011, India.
Bioresour Technol. 2016 Dec;222:458-469. doi: 10.1016/j.biortech.2016.10.012. Epub 2016 Oct 5.
A natural variant of Pyrenophora phaeocomes isolated from natural biodiversity was able to grow on various agricultural residues by co-producing laccase, xylanase and mannanase. Solid state fermentation of rice straw induced the highest productivities corresponding to 10,859.51±46.74, 22.01±1.00 and 10.45±0.128IUgds for laccase, xylanase and mannanase respectively after 4days. Besides producing the ligno-hemicellulolytic enzyme cocktail, 40days cultivation of P. phaeocomes S-1 on rice straw brought about the 63 and 51% degradation of lignin and hemicellulose. These components were further removed with mild alkali extraction revealing the overall losses amounting to 78 and 60% respectively for lignin, and hemicellulose. The biologically pretreated straw upon enzymatic hydrolysis revealed 50% saccharification efficiency releasing 470mgg sugars. Application of this knowledge will lead to efficient management of waste rice straw with low cost production of industrially important enzymes cocktail and its biological delignification for effective enzymatic hydrolysis to free sugars.
从天然生物多样性中分离出的 Pyrenophora phaeocomes 的天然变体能够通过共生产漆酶、木聚糖酶和甘露聚糖酶来生长在各种农业残留物上。稻草的固态发酵在 4 天后诱导了最高的产物，分别对应漆酶、木聚糖酶和甘露聚糖酶的 10,859.51±46.74、22.01±1.00 和 10.45±0.128IUgds。除了产生木质素-半纤维素酶混合物外，P. phaeocomes S-1 在稻草上培养 40 天导致木质素和半纤维素分别降解了 63%和 51%。用温和的碱提取进一步去除这些成分，木质素和半纤维素的总损失分别达到 78%和 60%。经过生物预处理的稻草在酶解后，释放出 470mgg 糖的糖化效率达到 50%。这项知识的应用将导致对废弃稻草的有效管理，以低成本生产工业上重要的酶混合物，并对其进行生物脱木质素处理，以实现有效酶解释放游离糖。
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