Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; Department of Biology, Lakehead University, Ontario P7B 5E1, Canada; Co-innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Department of Biology, Lakehead University, Ontario P7B 5E1, Canada.
Bioresour Technol. 2019 Feb;274:459-467. doi: 10.1016/j.biortech.2018.12.029. Epub 2018 Dec 10.
Based on a general understanding that hemicellulose removal is more efficient than delignification for biomass deconstruction, an Aspergillus niger strain producing high xylanase activity was screened out from seventeen strains by clear halo experiments. Low-cost enzyme cocktail with high xylanase activity was produced from wheat straw medium fermented by the Gyx086 strain. The enzyme cocktail with high xylanase activity could more effectively hydrolyze wheat straw than other biomasses. However, only 30% of total carbohydrates could be hydrolyzed to reducing sugar in untreated wheat straw. Further enzymatic hydrolysis and pretreated trials were carried out, the results indicated that hemicellulose removal was less effective than delignification for de-recalcitrance of wheat straw and the crystallinity is little interference with the hydrolysis process. Delignified wheat straw was near-completely hydrolyzed by the enzyme cocktail in 60 h. This study advanced the knowledge in promoting wheat straw as feedstock for bio-based industry.
基于半纤维素去除比木质素去除更有利于生物质解构的普遍认识，通过透明晕圈实验从 17 株菌中筛选出一株产木聚糖酶活性较高的黑曲霉。从 Gyx086 菌株发酵的小麦秸秆培养基中生产出低成本、高木聚糖酶活性的酶混合物。该高木聚糖酶活性的酶混合物比其他生物质更有效地水解小麦秸秆。然而，未经处理的小麦秸秆中只有 30%的总碳水化合物可水解为还原糖。进一步进行酶水解和预处理试验，结果表明，对于小麦秸秆的去抗生性，半纤维素去除的效果不如木质素去除，结晶度对水解过程的干扰很小。经脱木质素处理的小麦秸秆在 60 小时内被酶混合物近乎完全水解。本研究为促进小麦秸秆作为生物基产业原料提供了知识支持。
