Xu Aihua, Ahsanul Kabir Khan Md, Chen Fangzhi, Zhong Zhaohui, Chen Han-Chun, Song Yuanda
Colin Ratledge Center for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, People's Republic of China.
Department of Biochemistry, School of Life Sciences and The State Key Laboratory of Medical Genetics, Central South University, Changsha, 410013, Hunan, People's Republic of China.
Med Oncol. 2016 Nov;33(11):131. doi: 10.1007/s12032-016-0836-7. Epub 2016 Oct 18.
Autotaxin (ATX) as an important tumor cell motility-stimulating factor is upregulated in many different types of cancer. ATX, a member of the ectonucleotide pyrophosphatase and phosphodiesterase family of enzymes, possesses lysophospholipase D activity which hydrolyzes lysophosphatidylcholine to generate the potent tumor growth factor and mitogen lysophosphatidic acid (LPA). LPA acts on specific G-protein-coupled receptors, thereby regulating cell growth, migration, and survival. This study aimed to investigate the differences in gene expression pattern of ATX between cancerous and adjacent normal tissue of human renal cell carcinoma (RCC) and bladder carcinoma (BC) and find the correlation between ATX expression and clinicopathological features of both of these carcinomas. Both the RCC and BC tissues and with the adjacent normal tissues were collected. Immunohistochemistry and Western blotting analysis were used to detect the extent of ATX expression in all of these samples. Immunohistochemistry and Western blot analysis revealed that expression of ATX protein in carcinoma tissues is significantly higher than that in the adjacent normal tissues. Immunohistochemistry analysis showed that ATX is localized in cytoplasm. Western blotting analysis showed that ATX protein is expressed in both RCC and BC, and the expression levels were 69.5 and 48.0 %, respectively, higher in RCC and BC carcinoma tissue samples than in the adjacent normal tissues, which is consistent with the results of immunohistochemistry study. Thus, this study provided the evidence that ATX is highly expressed in both RCC and BC. Further research can be done to identify the diagnosis and treatment significance of both these carcinomas.
自分泌运动因子（ATX）作为一种重要的肿瘤细胞运动刺激因子，在许多不同类型的癌症中表达上调。ATX是核苷酸焦磷酸酶和磷酸二酯酶家族的成员，具有溶血磷脂酶D活性，可水解溶血磷脂酰胆碱生成强效肿瘤生长因子和有丝分裂原溶血磷脂酸（LPA）。LPA作用于特定的G蛋白偶联受体，从而调节细胞生长、迁移和存活。本研究旨在探讨人肾细胞癌（RCC）和膀胱癌（BC）癌组织与癌旁正常组织中ATX基因表达模式的差异，并寻找ATX表达与这两种癌症临床病理特征之间的相关性。收集了RCC和BC组织以及相应的癌旁正常组织。采用免疫组织化学和蛋白质印迹分析检测所有这些样本中ATX的表达程度。免疫组织化学和蛋白质印迹分析显示，癌组织中ATX蛋白的表达明显高于癌旁正常组织。免疫组织化学分析表明ATX定位于细胞质中。蛋白质印迹分析显示，ATX蛋白在RCC和BC中均有表达，RCC和BC癌组织样本中的表达水平分别比癌旁正常组织高69.5%和48.0%，这与免疫组织化学研究结果一致。因此，本研究提供了证据表明ATX在RCC和BC中均高表达。可进一步开展研究以确定这两种癌症的诊断和治疗意义。
