Xie C W, Lee P H, Douglass J, Crain B, Hong J S
Neuropharmacology Section, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Brain Res. 1989 Aug 21;495(1):156-60. doi: 10.1016/0006-8993(89)91230-4.
The effect of deep prepyriform cortex (DPC) kindling on the levels of prodynorphin mRNA (DYN mRNA) in rat hippocampus and striatum was examined under two different stimulation paradigms. Electrical stimulations were delivered to rats twice per day (slow kindling) or once every hour (fast kindling) until two consecutive stage 5 kindled seizures occurred. Animals were decapitated 24 h after reaching the second stage 5 seizure, and DYN mRNA levels in the brain were determined by RNA blot analysis. In the slow kindling model, the level of DYN mRNA in the hippocampus was reduced by 57%, whereas the level of striatal DYN mRNA was increased by 34% compared to sham-operated controls. Fast kindling induced a similar decrease in the DYN mRNA level in the hippocampus, but did not alter that in the striatum. These results, together with the previous report that kindling decreased dynorphin A(1-8) level in the hippocampus, suggest that electrical kindling decreases the biosynthesis of dynorphin peptides in the hippocampus and, in the slow DPC kindling model, also increases the gene expression of dynorphin in the striatum.
在两种不同的刺激模式下，研究了深部梨状前皮质（DPC）点燃对大鼠海马体和纹状体中前强啡肽原mRNA（DYN mRNA）水平的影响。每天对大鼠进行两次电刺激（慢点燃）或每小时进行一次电刺激（快点燃），直到连续出现两次5期点燃癫痫发作。在达到第二次5期癫痫发作后24小时将动物断头，通过RNA印迹分析测定脑中DYN mRNA水平。在慢点燃模型中，与假手术对照组相比，海马体中DYN mRNA水平降低了57%，而纹状体中DYN mRNA水平升高了34%。快点燃诱导海马体中DYN mRNA水平出现类似下降，但未改变纹状体中的水平。这些结果，连同之前关于点燃降低海马体中强啡肽A（1-8）水平的报告，表明电点燃降低了海马体中强啡肽肽的生物合成，并且在慢DPC点燃模型中，还增加了纹状体中强啡肽的基因表达。
