Morris B J, Moneta M E, ten Bruggencate G, Höllt V
Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G.
Neurosci Lett. 1987 Oct 5;80(3):298-302. doi: 10.1016/0304-3940(87)90471-x.
The effect of hippocampal kindling on the levels of prodynorphin mRNA in rat hippocampus was examined by in situ hybridization using a synthetic oligonucleotide probe. Cryostat tissue sections were hybridised with a 32P-labelled 100 mer DNA probe complementary to the coding region of rat prodynorphin mRNA, and exposed to X-ray film. In rats exhibiting stage 4 seizures, the levels of prodynorphin mRNA in the dentate gyrus were dramatically reduced compared to control animals. This suggests that the development of kindling is accompanied by a reduction in the rate of synthesis of peptides derived from prodynorphin.
采用合成寡核苷酸探针，通过原位杂交技术检测海马点燃对大鼠海马中前强啡肽原mRNA水平的影响。用与大鼠前强啡肽原mRNA编码区互补的32P标记的100聚体DNA探针与恒冷箱组织切片杂交，并将其置于X线胶片上曝光。与对照动物相比，处于4期癫痫发作的大鼠齿状回中前强啡肽原mRNA水平显著降低。这表明点燃的发展伴随着源自前强啡肽原的肽合成速率的降低。
