Göder Anja, Mahendrarajah Nisintha, Krämer Oliver H
Institute of Toxicology, University Medical Center Mainz, 55131, Mainz, Germany.
Methods Mol Biol. 2017;1510:3-10. doi: 10.1007/978-1-4939-6527-4_1.
Autophagy is a lysosome-dependent, intracellular pathway for the recycling of cellular components. It plays a pivotal role in both cancer development and the response to chemotherapy. Here we describe how autophagy can be monitored in living cells by flow cytometry using the cationic amphiphilic tracer dye Cyto-ID Green. The detection of autophagy induction in the human leukemia cell line K562 after the treatment with the HDAC class I inhibitor MS-275 serves as an example for this approach.
自噬是一种依赖溶酶体的细胞内途径，用于细胞成分的循环利用。它在癌症发展和化疗反应中都起着关键作用。在此，我们描述了如何使用阳离子两亲性示踪染料Cyto-ID Green通过流式细胞术在活细胞中监测自噬。用I类组蛋白去乙酰化酶抑制剂MS-275处理后人白血病细胞系K562中自噬诱导的检测作为该方法的一个例子。
