Hungarian Satellite Centre of Trace Elements Institute to UNESCO, Pázmány Péter stny 1/A, Budapest, 1117, Hungary.
Laboratory of Environmental Chemistry and Bioanalytics, Department of Analytical Chemistry, Institute of Chemistry, Eötvös Loránd University, Pázmány Péter stny 1/A, Budapest, 1117, Hungary.
Environ Sci Pollut Res Int. 2018 Jan;25(3):2021-2034. doi: 10.1007/s11356-016-7787-6. Epub 2016 Oct 20.
The bulk analysis of single feathers of 263 feathers belonging to 238 individuals of a migratory passerine (collared flycatcher, Ficedula albicollis, originating from a breeding population in the Pilis-Visegrád Mountains in Hungary) by inductively coupled plasma sector field mass spectrometry (ICP-SF-MS) for determination of elements after proper dissolution allowed the quantitative determination of 38 elements. Calcium, Mg, Mn, Fe, and Zn were found to have a quantitative determination frequency larger than 80 % and a concentration greater than 100 μg/g. Among ecotoxicologically relevant elements, Ni, Cd, Hg, and Pb could be determined in more than 55 % of the tail feather samples. The concentration of Hg with a quantification limit of 0.006 μg/g and Pb with that of 0.015 μg/g was higher than 1 and 10 μg/g, respectively, in more than 80 % of the investigated samples, but generally lower than levels that could cause adverse behavioral effects. The principal component analyses of elemental concentration data followed by the application of general linear models revealed that, for male collared flycatchers, the concentration of Sn, Pb, Ni, Sr, Mg, Zn, Ba, and Sc differed significantly in the wing and tail feathers collected from the same individuals. With females, only the Ca and Sc concentration showed a significant difference between wing and tail feathers. Moreover, the concentration of rare earth elements, V, Fe, Sr, Mg, Mn, Zn, Pb, and Ba in tail feathers allowed differentiation between sexes while the concentration of Se, Bi, and Sc between yearling and adult male individuals. At the same time, Sc differentiated age categories in females. Distribution of major elements along the rachis of feathers could be monitored by laser ablation ICP-SF-MS after normalization of the intensities to either C or S signals.
采用电感耦合等离子体质谱（ICP-SF-MS）对 238 只迁徙雀形目鸟类（领雀嘴鹎，Ficedula albicollis，来自匈牙利皮利什-维谢格拉德山脉的繁殖种群）的 263 根羽毛的单个羽毛进行批量分析，对羽毛进行适当溶解后，可测定 38 种元素。发现钙、镁、锰、铁和锌的定量测定频率大于 80%，浓度大于 100μg/g。在具有生态毒理学意义的元素中，超过 55%的尾羽样本可测定出镍、镉、汞和铅。痕量汞（定量限为 0.006μg/g）和痕量铅（定量限为 0.015μg/g）的浓度在超过 80%的研究样本中分别高于 1μg/g 和 10μg/g，但通常低于可能引起不良行为影响的水平。对元素浓度数据进行主成分分析，然后应用一般线性模型，结果表明，对于雄性领雀嘴鹎，来自同一个体的翅膀和尾羽的元素浓度在锡、铅、镍、锶、镁、锌、钡和钪方面存在显著差异。对于雌性，只有钙和钪的浓度在翅膀和尾羽之间存在显著差异。此外，尾羽中的稀土元素、钒、铁、锶、镁、锰、锌、铅和钡的浓度可以区分性别，而硒、铋和钪的浓度可以区分幼鸟和成鸟雄性个体。同时，钪可以区分雌性的年龄类别。通过激光烧蚀 ICP-SF-MS 对羽毛羽轴上的主要元素进行监测，可通过将强度归一化为 C 或 S 信号进行监测。
