Sebbag Lionel, Park Shin Ae, Kass Philip H, Maggs David J, Attar Mayssa, Murphy Christopher J
William R. Pritchard Veterinary Medical Teaching Hospital, University of California, Davis, CA, USA.
Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
Vet Ophthalmol. 2017 Jul;20(4):357-364. doi: 10.1111/vop.12436. Epub 2016 Oct 20.
To evaluate repeatability and reproducibility of tear osmolarity measured using the TearLab osmometer in normal dogs and to assess its diagnostic potential in dogs with keratoconjunctivitis sicca (KCS).
Beagle dogs; six normal and five with KCS.
Tear osmolarity and Schirmer tear test-1 (STT-1) values were obtained at various times. Normal dogs were assessed for diurnal variation and repeatability and reproducibility of measurements. Dogs with KCS were evaluated before and after 5 months' topical twice-daily therapy with 2% cyclosporine.
Mean ± SD tear osmolarity (mOsm/L) was significantly higher in normal dogs (337.4 ± 16.2) than in dogs with KCS before therapy (306.2 ± 18.0; P < 0.0001), but not following therapy with 2% cyclosporine (330.5 ± 13.7; P = 1.00). Osmolarity readings lower than 325.5 mOsm/L were suggestive of KCS (84.8% sensitivity and 87.1% specificity). In normal dogs, tear osmolarity readings were stable during the daytime (P = 0.99). Repeated measurements revealed high variability and typically poor-to-moderate repeatability and reproducibility, although this was improved by taking three successive measurements at each session. Considering combined data from all dogs, a positive correlation existed between STT-1 and tear osmolarity measurements (Pearson's correlation test, P = 0.04, r = 0.62).
Canine tear osmolarity as determined by TearLab osmometer was variable, required multiple readings to be informative, and differed from values reported for humans. Dogs with KCS had a lower tear osmolarity than did normal dogs, and this increased following cyclosporine therapy.
评估使用TearLab渗透压计测量正常犬泪液渗透压的重复性和再现性，并评估其在患有干眼症（KCS）的犬中的诊断潜力。
比格犬；6只正常犬和5只患有KCS的犬。
在不同时间获取泪液渗透压和泪液分泌试验-1（STT-1）值。评估正常犬的昼夜变化以及测量的重复性和再现性。对患有KCS的犬在每天两次局部使用2%环孢素治疗5个月前后进行评估。
正常犬的平均±标准差泪液渗透压（mOsm/L）（337.4±16.2）显著高于治疗前患有KCS的犬（306.2±18.0；P<0.0001），但在使用2%环孢素治疗后（330.5±13.7；P = 1.00）则无显著差异。渗透压读数低于325.5 mOsm/L提示患有KCS（敏感性84.8%，特异性87.1%）。在正常犬中，白天泪液渗透压读数稳定（P = 0.99）。重复测量显示变异性高，通常重复性和再现性较差至中等，不过每次测量时连续进行三次测量可改善这种情况。综合所有犬的数据来看，STT-1与泪液渗透压测量值之间存在正相关（Pearson相关检验，P = 0.04，r = 0.62）。
通过TearLab渗透压计测定的犬泪液渗透压具有变异性，需要多次读数才能提供有效信息，且与人类报告的值不同。患有KCS的犬泪液渗透压低于正常犬，环孢素治疗后该数值升高。
