使用针对通过简便方法获得的抗原复合物产生的抗血清对2-羟雌酮进行放射免疫测定。

Radioimmunoassay of 2-hydroxyesterone using antisera raised against antigenic complexes obtained by convenient methods.

作者信息

Numazawa M, Kimura K, Nagaoka M, Kambegawa A

出版信息

Chem Pharm Bull (Tokyo). 1989 Jun;37(6):1561-3. doi: 10.1248/cpb.37.1561.

DOI:10.1248/cpb.37.1561

Abstract

Antigenic complexes of 2-hydroxyestrone (2-OHE1) were obtained by Mannich reaction of 2-OHE1 and bovine serum albumin (BSA) and by coupling of 2-OHE1 1-glutathione thioether to BSA using glutaraldehyde. Antiserum raised against the antigen obtained by the Mannich reaction had high affinity (Kd = 3.8 x 10(9) M-1) and relatively high specificity; cross reactivities for estrone, 4-hydroxyestrone and 2-methoxyestrone were 2.1%, 10% and 1.5%, respectively. The other antiserum also had high affinity (4.5 x 10(9) M-1) but its cross reactivities for the above three steroids were more than 100%. Concentrations of 2-hydroxyestrone in human plasma were determined by radioimmunoassay with the more specific antiserum and Sephadex LH-20 chromatography to be less than a minimum detectable amount (less than 10 pg/ml) (men), 20.9 pg/ml (women, proliferation) and 26.0 pg/ml (women, periovulation).

摘要

通过2-羟雌酮（2-OHE1）与牛血清白蛋白（BSA）的曼尼希反应以及使用戊二醛将2-OHE1 1-谷胱甘肽硫醚与BSA偶联，获得了2-羟雌酮的抗原复合物。针对通过曼尼希反应获得的抗原产生的抗血清具有高亲和力（Kd = 3.8 x 10⁹ M⁻¹）和相对较高的特异性；雌酮、4-羟雌酮和2-甲氧基雌酮的交叉反应率分别为2.1%、10%和1.5%。另一种抗血清也具有高亲和力（4.5 x 10⁹ M⁻¹），但其对上述三种甾体的交叉反应率超过100%。使用更具特异性的抗血清和葡聚糖凝胶LH-20色谱法通过放射免疫测定法测定人血浆中2-羟雌酮的浓度，结果为低于最低可检测量（低于10 pg/ml）（男性）、20.9 pg/ml（女性，增殖期）和26.0 pg/ml（女性，排卵期）。