Zainal Abidin Syafiq Asnawi, Rajadurai Pathmanathan, Chowdhury Md Ezharul Hoque, Ahmad Rusmili Muhamad Rusdi, Othman Iekhsan, Naidu Rakesh
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, Malaysia.
Ramsay Sime Darby Healthcare, Sime Darby Medical Centre, No. 1, Jalan SS12/1A, Subang Jaya, Selangor Darul Ehsan 47500, Malaysia.
Toxins (Basel). 2016 Oct 18;8(10):299. doi: 10.3390/toxins8100299.
and are venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins in and . They were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. Enzymatic families detected in and venom were snake venom metalloproteinase, phospholipase A₂, ʟ-amino acid oxidase, serine proteases, 5'-nucleotidase, phosphodiesterase, and phospholipase B. In addition, glutaminyl cyclotransferase was detected in . C-type lectin-like proteins were common nonenzymatic components in both species. Waglerin was present and unique to -it was not in venom. In contrast, cysteine-rich secretory protein, bradykinin-potentiating peptide, and C-type natriuretic peptide were present in venom. Composition of the venom proteome of and provides useful information to guide production of effective antivenom and identification of proteins with potential therapeutic applications.
[具体蛇名1]和[具体蛇名2]是马来西亚常见的有毒蝰蛇种类。对其粗毒进行串联质谱分析，在[具体蛇名1]和[具体蛇名2]中检测到了不同的蛋白质。它们被分为13个毒蛋白家族，包括酶蛋白和非酶蛋白。在[具体蛇名1]和[具体蛇名2]的毒液中检测到的酶家族有蛇毒金属蛋白酶、磷脂酶A₂、L-氨基酸氧化酶、丝氨酸蛋白酶、5'-核苷酸酶、磷酸二酯酶和磷脂酶B。此外，在[具体蛇名1]中检测到了谷氨酰胺环化转移酶。C型凝集素样蛋白是这两种蛇毒液中常见的非酶成分。瓦格勒毒素存在于[具体蛇名1]毒液中且是其特有的——[具体蛇名2]毒液中没有。相反，富含半胱氨酸的分泌蛋白、缓激肽增强肽和C型利钠肽存在于[具体蛇名2]毒液中。[具体蛇名1]和[具体蛇名2]毒液蛋白质组的组成提供了有用信息，以指导有效抗蛇毒血清的生产以及鉴定具有潜在治疗应用的蛋白质。
