Harada T, Hobby P, Courteau M, Knight J F, Williams D G
Renal Unit, United Medical School of Guy's, London, England.
Clin Exp Immunol. 1989 Aug;77(2):211-4.
The spectrotype of plasma IgA in patients with IgA nephropathy was studied by isoelectric focussing. Densitometry of the gels showed a significant increase in the anionic region at isoelectric points (pI) 4.7-5.1 (P = 0.02) and a reduction in the cationic region pI 5.8-6.0 (P = 0.03) in patients (n = 15) compared with controls (n = 8). Measurement of the IgA concentration in eluates of sequential slices of the gels showed that the ratio of anionic-to-cationic IgA, using pI 5.6 as the dividing point, was significantly greater in patients (n = 10) than in controls (n = 10) (P = 0.03). Two different methods of analysis have therefore demonstrated an increased proportion of anionic and decreased proportion of cationic IgA in the plasma of patients with IgA nephropathy.
采用等电聚焦法研究了IgA肾病患者血浆IgA的光谱类型。凝胶密度测定显示，与对照组（n = 8）相比，患者组（n = 15）在等电点（pI）4.7 - 5.1的阴离子区域显著增加（P = 0.02），而在pI 5.8 - 6.0的阳离子区域减少（P = 0.03）。对凝胶连续切片洗脱液中IgA浓度的测量表明，以pI 5.6为分界点，患者组（n = 10）的阴离子IgA与阳离子IgA之比显著高于对照组（n = 10）（P = 0.03）。因此，两种不同的分析方法均表明IgA肾病患者血浆中阴离子IgA比例增加，阳离子IgA比例降低。
