IgA肾病患者肾小球中聚合IgA的检测。
Detection of polymeric IgA in glomeruli from patients with IgA nephropathy.
作者信息
Tomino Y, Sakai H, Miura M, Endoh M, Nomoto Y
出版信息
Clin Exp Immunol. 1982 Aug;49(2):419-25.
A study on the detection of polymeric IgA in glomeruli from renal biopsy specimens in patients with IgA nephropathy is described. Renal biopsy specimens were obtained from patients with IgA nephropathy. These specimens were stained with FITC-labelled anti-human J chain antisera and then examined with a fluorescent microscope. The J chain was observed in the glomerular mesangium by immunofluorescent staining. In parallel studies, renal biopsy specimens were treated with citrate buffer (pH 3.2) and the 'eluate' was neutralized by sodium hydroxide. The eluate was labelled with iodine-125, and the radiolabelled 'eluate' was fractionated by sucrose density-gradient ultracentrifugation. Polymerized IgA in the 'eluate' obtained from patients with IgA nephropathy was found to sediment predominantly as 9S to 11S using a sucrose density gradient analysis. Polymeric IgA in the fractions of the density gradient analysis was determined by anti-human IgA and anti-human J chain antisera. It was demonstrated that IgA and J chain were eluted from the glomeruli in some patients with IgA nephropathy. It is concluded that IgA deposited in the glomeruli is composed of dimers and/or larger polymers of circulating IgA in some patients with IgA nephropathy.
本文描述了一项关于检测IgA肾病患者肾活检标本肾小球中聚合IgA的研究。肾活检标本取自IgA肾病患者。这些标本用异硫氰酸荧光素（FITC）标记的抗人J链抗血清染色，然后用荧光显微镜检查。通过免疫荧光染色在肾小球系膜中观察到J链。在平行研究中，肾活检标本用柠檬酸盐缓冲液（pH 3.2）处理，“洗脱液”用氢氧化钠中和。洗脱液用碘-125标记，放射性标记的“洗脱液”通过蔗糖密度梯度超速离心进行分级分离。使用蔗糖密度梯度分析发现，从IgA肾病患者获得的“洗脱液”中的聚合IgA主要以9S至11S的形式沉淀。密度梯度分析各组分中的聚合IgA通过抗人IgA和抗人J链抗血清进行测定。结果表明，在一些IgA肾病患者中，IgA和J链从肾小球中洗脱出来。得出的结论是，在一些IgA肾病患者中，沉积在肾小球中的IgA由循环IgA的二聚体和/或更大的聚合物组成。
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