使用蓝色和红色激光对Dendra2进行从绿色到红色的引发转换。

Green-to-red primed conversion of Dendra2 using blue and red lasers.

作者信息

Klementieva N V, Lukyanov K A, Markina N M, Lukyanov S A, Zagaynova E V, Mishin A S

机构信息

Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.

出版信息

Chem Commun (Camb). 2016 Nov 18;52(89):13144-13146. doi: 10.1039/c6cc05599k. Epub 2016 Oct 20.

DOI:10.1039/c6cc05599k

PMID:27763646

Abstract

Recently, an unusual phenomenon of primed conversion of fluorescent protein Dendra2 by combined action of blue (488 nm) and near-infrared (700-780 nm) lasers was discovered. Here we demonstrate that primed conversion can be induced by red lasers (630-650 nm) common for most confocal and single molecule detection microscopes.

摘要

最近，人们发现了一种不寻常的现象，即荧光蛋白Dendra2在蓝色（488纳米）和近红外（700 - 780纳米）激光的联合作用下发生预转换。在此我们证明，预转换可以由大多数共聚焦和单分子检测显微镜常用的红色激光（630 - 650纳米）诱导产生。