Chow Renee Wei-Yan, Vermot Julien
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
Centre National de la Recherche Scientifique UMR8104, Illkirch, France.
F1000Res. 2017 Apr 11;6. doi: 10.12688/f1000research.10617.1. eCollection 2017.
The zebrafish ( ) is a powerful vertebrate model to study cellular and developmental processes . The optical clarity and their amenability to genetic manipulation make zebrafish a model of choice when it comes to applying optical techniques involving genetically encoded photoresponsive protein technologies. In recent years, a number of fluorescent protein and optogenetic technologies have emerged that allow new ways to visualize, quantify, and perturb developmental dynamics. Here, we explain the principles of these new tools and describe some of their representative applications in zebrafish.
斑马鱼是研究细胞和发育过程的强大脊椎动物模型。其光学透明度以及对基因操作的易处理性，使得斑马鱼成为应用涉及基因编码光响应蛋白技术的光学技术时的首选模型。近年来，出现了许多荧光蛋白和光遗传学技术，这些技术为可视化、量化和干扰发育动态提供了新方法。在此，我们解释这些新工具的原理，并描述它们在斑马鱼中的一些代表性应用。
