Chhabria Vikesh, Beeton Steve
School of Pharmacy & Biomedical Sciences, University of Central Lancashire, Preston, Lancashire, PR1 2HE, UK.
Nanomedicine (Lond). 2016 Nov;11(21):2797-2807. doi: 10.2217/nnm-2016-0180. Epub 2016 Oct 21.
To produce mammalian biomimetic nanosponges from mammalian erythrocyte ghosts. Biomimetic nanosponges were studied in vitro as treatment platforms against exotoxin-related sepsis.
Ovine blood was treated with hypotonic buffer to create erythrocyte ghosts and then subjected to sonication to produce erythrocyte vesicles of nonuniform size. Vesicles were then serially extruded through 400-nm and 100-nm polycarbonate membranes. Nanosponges were prepared by fusing poly(d,l-lactic-co-glycolic acid) cores with ovine erythrocyte vesicles.
Ovine erythrocytes were the most susceptible to streptolysin-O lysis, making it a model to study sepsis treatment. Ovine nanosponges adsorbed streptolysin-O at 37 and 40°C.
These results identify ovine nanosponges as novel therapeutic model to test adsorption of cholesterol binding toxins such as streptolysin-O.
从哺乳动物红细胞影体制备哺乳动物仿生纳米海绵。对仿生纳米海绵进行体外研究，作为抗外毒素相关败血症的治疗平台。
用低渗缓冲液处理绵羊血液以制备红细胞影，然后进行超声处理以产生大小不均匀的红细胞囊泡。然后将囊泡依次通过400纳米和100纳米的聚碳酸酯膜挤出。通过将聚（d，l-乳酸-共-乙醇酸）核与绵羊红细胞囊泡融合来制备纳米海绵。
绵羊红细胞对链球菌溶血素-O裂解最敏感，使其成为研究败血症治疗的模型。绵羊纳米海绵在37和40°C下吸附链球菌溶血素-O。
这些结果确定绵羊纳米海绵是测试胆固醇结合毒素如链球菌溶血素-O吸附的新型治疗模型。
