Yazdekhasti Hossein, Rajabi Zahra, Parvari Soraya, Abbasi Mehdi
Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
J Ovarian Res. 2016 Oct 20;9(1):68. doi: 10.1186/s13048-016-0274-3.
Although existence of ovarian stem cells (OSCs) in mammalian postnatal ovary is still under controversy, however, it has been almost accepted that OSCs are contributing actively to folliculogenesis and neo-oogenesis. Recently, various methods with different efficacies have been employed for OSCs isolation from ovarian tissue, which these methods could be chosen depends on aim of isolation and accessible equipments and materials in lab. Although isolated OSCs from different methods have various traits and characterizations, which might become from their different nature and origin, however these stem cells are promising source for woman infertility treatment or source of energy for women with a history of repeat IVF failure in near future. This review has brought together and summarized currently used protocols for isolation and propagation of OSCs in vitro.
尽管哺乳动物出生后卵巢中卵巢干细胞（OSCs）的存在仍存在争议，但几乎已经公认OSCs在卵泡发生和新卵泡形成中发挥着积极作用。最近，已采用各种效率不同的方法从卵巢组织中分离OSCs，选择这些方法取决于分离目的以及实验室可用的设备和材料。尽管通过不同方法分离的OSCs具有各种特性和特征，这可能源于它们不同的性质和来源，但这些干细胞有望成为治疗女性不孕症的来源，或在不久的将来为有多次体外受精失败史的女性提供能量来源。本综述汇集并总结了目前用于体外分离和扩增OSCs的方案。
