Ke B X, He D M, Tan H L, Zeng H H, Yang T, Li B S, Liang Y H, Lu L L, Liang J H, Huang Q, Ke C W
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
Guangdong Provincial Institute of Biological Products and Material Medicine, Guangzhou, 510440, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2016 Oct 10;37(10):1373-1378. doi: 10.3760/cma.j.issn.0254-6450.2016.10.012.
To study the infection status, serotypes, drug resistance and molecular characteristics of , enterotoxigenic () (ETEC), pathogenic (EPEC), Shiga Toxin producing (STEC) and Enteroinvasive (EIEC) collected from diarrhea patients in Guangdong. The strains of and 4 kinds of isolated from foodborne diseases surveillance during 2013-2014 were collected to conduct serotyping, drug resistance test and pulsed-field gel electrophoresis (PFGE). A total of 3 372 stains of pathogens were isolated from 57 834 stool samples during 2013-2014, the overall positive rate was 5.83 and the positive rate of was highest, followed by that of , 4 kinds of and . And 3 213 strains of were divided into 143 serotypes. The most prevalent serotypes were , 4, 5, 12: i:-, , and . was sensitive to cephalosporin and fluoroquinolones, and showed significant differences in drug resistance rate among different serotypes. In top 10 common serotypes, and were most resistant to cephalosporin and ciprofloxacin respectively. PFGE was performed for 2 289 strains of , showing distribution diversity and significant fingerprint polymorphisms. The 85 strains of were divided into 10 serotypes, O3∶K6 (61.18) was the most common serotype, followed by O4∶K8. The results showed that the carrying rate of virulence genes (81.18) was high, while the carrying rate of was low (7.06), and there were 10 strains carrying no the two kinds of virulence genes. The sensitive rate of to imipenem, nalidixic acid, SMZ-TMP, chloramphenicol and tetracycline were more than 95. Thirteen strains of were detected, including 9 strains of , 3 strains of and 1 strains of . The strains all showed sensitivity to ceftazidime, ciprofloxacin and chloramphenicol (76.92). There were 86 strains of detected, including 29 strains of ETEC (33.72), 27 strains of EPEC (31.39), 27 strains of STEC (31.39) and 3 strains of EIEC (3.48). In the active etiological surveillance for foodborne diseases in Guangdong during 2013-2014, the detection rate of was highest (5.57 ), followed by that of , 4 kinds of and . , and were sensitive to cephalosporin and fluoroquinolones. Clustered cases of infection were found in the surveillance, but no outbreaks occurred.
为研究从广东腹泻患者中分离出的肠产毒性大肠埃希菌（ETEC）、致病性大肠埃希菌（EPEC）、产志贺毒素大肠埃希菌（STEC）和侵袭性大肠埃希菌（EIEC）的感染状况、血清型、耐药性及分子特征。收集2013 - 2014年食源性疾病监测中分离出的大肠埃希菌及4种致泻性大肠埃希菌菌株进行血清分型、耐药性检测及脉冲场凝胶电泳（PFGE）分析。2013 - 2014年期间，从57834份粪便样本中共分离出3372株病原菌，总体阳性率为5.83%，大肠埃希菌阳性率最高，其次为4种致泻性大肠埃希菌。3213株大肠埃希菌分为143个血清型，最常见的血清型为O1、4、5、12∶i∶ - 、O25、O157和O111。大肠埃希菌对头孢菌素和氟喹诺酮类敏感，不同血清型耐药率存在显著差异，前10种常见血清型中，O1和O157分别对头孢菌素和环丙沙星耐药性最强。对2289株大肠埃希菌进行PFGE分析，显示出分布多样性和明显指纹多态性。85株致泻性大肠埃希菌分为10个血清型，O3∶K6（61.18%）为最常见血清型，其次为O4∶K8。结果显示，毒力基因stx的携带率较高（81.18%），eae的携带率较低（7.06%），有10株未携带这两种毒力基因。大肠埃希菌对亚胺培南、萘啶酸、复方磺胺甲恶唑、氯霉素和四环素的敏感率均超过95%。检测到13株EIEC，包括9株O124、3株O164和1株O152。这些菌株对头孢他啶、环丙沙星和氯霉素均敏感（76.92%）。检测到86株致泻性大肠埃希菌，包括29株ETEC（33.72%）、27株EPEC（31.39%）、27株STEC（31.39%）和3株EIEC（3.48%）。在2013 - 2014年广东食源性疾病主动病因监测中，大肠埃希菌检出率最高（5.57%），其次为4种致泻性大肠埃希菌。大肠埃希菌、ETEC和EPEC对头孢菌素和氟喹诺酮类敏感。监测中发现大肠埃希菌感染聚集性病例，但未发生暴发。
