Kimron Veterinary Institute, Department of Toxicology, Bet Dagan 50250, Israel.
Kimron Veterinary Institute, Department of Toxicology, Bet Dagan 50250, Israel.
Food Chem. 2017 Mar 15;219:459-467. doi: 10.1016/j.foodchem.2016.09.182. Epub 2016 Sep 29.
Sorghum ergot is a disease caused commonly by C. africana. In 2015, ergot was identified for the first time in sorghum fields in Israel, leading to measures of eradication and quarantine. The aims of the study were to identify the ergot species by molecular and ergot alkaloid profile analysis, to determine the ergot alkaloid profile in pure honeydew and in infected sorghum silages and to estimate the safety of sorghum silages as a feed source. C. africana was rapidly and reliably identified by microscopical and molecular analysis. Dihydroergosine was identified as the major ergot alkaloid. Dihydrolysergol and dihydroergotamine were identified for the first time as significant ergot alkaloid components within the C. africana sclerotia, thereby providing for the first time a proof for the natural occurrence of dihydroergotamine. The sorghum silages were found to be safe for feed consumption, since the ergot alkaloids and the regulated mycotoxins were below their regulated limits.
高粱麦角病通常由 C. africana 引起。2015 年，以色列首次在高粱田中发现了麦角病，随后采取了根除和检疫措施。本研究的目的是通过分子和麦角生物碱分析鉴定麦角菌种，确定纯蜜露和感染的高粱青贮中麦角生物碱的含量，并评估高粱青贮作为饲料来源的安全性。通过显微镜和分子分析，快速可靠地鉴定出 C. africana。鉴定出二氢麦角辛为主要的麦角生物碱。首次鉴定出二羟色胺和二氢麦角胺是 C. africana 菌核中的重要麦角生物碱成分，从而首次证明了二氢麦角胺的天然存在。由于麦角生物碱和受监管的霉菌毒素含量低于规定的限量，因此这些高粱青贮可以安全用于饲料。
