Zhang Jing, Wang You, Sun Kai-Ming, Fang Kuan, Tang Xuexi
Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Mar Pollut Bull. 2016 Dec 15;113(1-2):408-413. doi: 10.1016/j.marpolbul.2016.10.032. Epub 2016 Oct 17.
Polybrominated diphenyl ethers (PBDEs) are widely dispersed persistent organic pollutants in the marine ecosystem. However, their toxic mechanisms in marine organisms, especially invertebrates, remain poorly understood. Two common congeners of PBDEs, tetrabrominated diphenyl ether-47 (BDE-47) and decabrominated diphenyl ether-209 (BDE-209), were investigated. Their toxic mechanisms, with a focus on oxidative stress, were examined in rotifer Brachionus plicatilis. Overproduction of reactive oxygen species (ROS) was induced by two PBDEs. The expression of superoxide dismutase (SOD) mRNA was increased, suggesting SOD play a main role in ROS-scavenging. The intercellular concentrations of calcium ([Ca]) and the expression of calmodulin (CaM) mRNA were increased. This indicates the calcium ion (Ca) signaling channel is involved in PBDEs stress. Further analysis showed that the reproductive system might be the target site for toxicity of PBDEs. Moreover, high value of detection indexes in BDE-47 experimental groups suggested BDE-47 might cause higher oxidative damage than BDE-209 in rotifers.
多溴二苯醚（PBDEs）是海洋生态系统中广泛分布的持久性有机污染物。然而，它们在海洋生物尤其是无脊椎动物中的毒性机制仍知之甚少。研究了PBDEs的两种常见同系物，四溴二苯醚-47（BDE-47）和十溴二苯醚-209（BDE-209）。在褶皱臂尾轮虫中研究了它们以氧化应激为重点的毒性机制。两种PBDEs均诱导了活性氧（ROS）的过量产生。超氧化物歧化酶（SOD）mRNA的表达增加，表明SOD在清除ROS中起主要作用。细胞内钙离子浓度（[Ca]）和钙调蛋白（CaM）mRNA的表达增加。这表明钙离子（Ca）信号通道参与了PBDEs应激。进一步分析表明，生殖系统可能是PBDEs毒性的靶位点。此外，BDE-47实验组的检测指标值较高，表明BDE-47在轮虫中可能比BDE-209造成更高的氧化损伤。
