Carr Martin, Richter Daniel J, Fozouni Parinaz, Smith Timothy J, Jeuck Alexandra, Leadbeater Barry S C, Nitsche Frank
School of Applied Sciences, University of Huddersfield, Huddersfield HD1 3DH, UK.
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
Mol Phylogenet Evol. 2017 Feb;107:166-178. doi: 10.1016/j.ympev.2016.10.011. Epub 2016 Oct 17.
Recent studies have shown that molecular phylogenies of the choanoflagellates (Class Choanoflagellatea) are in disagreement with their traditional taxonomy, based on morphology, and that Choanoflagellatea requires considerable taxonomic revision. Furthermore, phylogenies suggest that the morphological and ecological evolution of the group is more complex than has previously been recognized. Here we address the taxonomy of the major choanoflagellate order Craspedida, by erecting four new genera. The new genera are shown to be morphologically, ecologically and phylogenetically distinct from other choanoflagellate taxa. Furthermore, we name five novel craspedid species, as well as formally describe ten species that have been shown to be either misidentified or require taxonomic revision. Our revised phylogeny, including 18 new species and sequence data for two additional genes, provides insights into the morphological and ecological evolution of the choanoflagellates. We examine the distribution within choanoflagellates of these two additional genes, EF-1A and EFL, closely related translation GTPases which are required for protein synthesis. Mapping the presence and absence of these genes onto the phylogeny highlights multiple events of gene loss within the choanoflagellates.
最近的研究表明，领鞭毛虫（领鞭毛虫纲）的分子系统发育与其基于形态学的传统分类法不一致，并且领鞭毛虫纲需要进行大量的分类修订。此外，系统发育表明该类群的形态和生态进化比之前所认识到的更为复杂。在此，我们通过建立四个新属来探讨主要领鞭毛虫目有领鞭毛虫目的分类。新属在形态、生态和系统发育上均与其他领鞭毛虫类群不同。此外，我们命名了五个新的有领鞭毛虫物种，并正式描述了十个已被证明是鉴定错误或需要分类修订的物种。我们修订后的系统发育，包括18个新物种以及另外两个基因的序列数据，为领鞭毛虫的形态和生态进化提供了见解。我们研究了另外两个基因EF - 1A和EFL在领鞭毛虫中的分布情况，这两个基因是密切相关的翻译GTP酶，是蛋白质合成所必需的。将这些基因的有无情况映射到系统发育树上，突出了领鞭毛虫内部基因丢失的多个事件。
