Matsumura Yasufumi, Pitout Johann D D, Gomi Ryota, Matsuda Tomonari, Noguchi Taro, Yamamoto Masaki, Peirano Gisele, DeVinney Rebekah, Bradford Patricia A, Motyl Mary R, Tanaka Michio, Nagao Miki, Takakura Shunji, Ichiyama Satoshi
Emerg Infect Dis. 2016 Nov;22(11):1900-1907. doi: 10.3201/eid2211.160519.
The Escherichia coli sequence type (ST) 131 C2/H30Rx clade with the bla gene had been most responsible for the global dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. ST131 C1/H30R with bla emerged among ESBL-producing E. coli in Japan during the late 2000s. To investigate the possible expansion of a single clade we performed whole-genome sequencing for 43 Japan and 10 global ST131 isolates with bla (n = 16), bla (n = 16), bla (n = 13), and others (n = 8). We also included 8 ST131 genomes available in public databases. Core genome-based analysis of 61 isolates showed that ST131 with bla from 5 countries formed a distinct cluster within the C1/H30R clade, named C1-M27 clade. Accessory genome analysis identified a unique prophage-like region, supporting C1-M27 as a distinct clade. Our findings indicate that the increase of ESBL-producing E. coli in Japan is due mainly to emergence of the C1-M27 clade.
携带bla基因的大肠杆菌序列类型（ST）131 C2/H30Rx进化枝是产超广谱β-内酰胺酶（ESBL）大肠杆菌在全球传播的主要原因。携带bla的ST131 C1/H30R在21世纪末出现在日本产ESBL的大肠杆菌中。为了研究单个进化枝可能的扩张情况，我们对43株日本分离株和10株全球携带bla（n = 16）、bla（n = 16）、bla（n = 13）及其他bla（n = 8）的ST131分离株进行了全基因组测序。我们还纳入了公共数据库中可获取的8个ST131基因组。对61株分离株进行的基于核心基因组的分析表明，来自5个国家的携带bla的ST131在C1/H30R进化枝内形成了一个独特的簇，命名为C1-M27进化枝。辅助基因组分析鉴定出一个独特的前噬菌体样区域，支持C1-M27作为一个独特的进化枝。我们的研究结果表明，日本产ESBL大肠杆菌的增加主要是由于C1-M27进化枝的出现。
