Kawayanagi Tomoki, Kawada-Matsuo Miki, Takeshita Toru, Nguyen-Tra Le Mi, Asakawa Mikari, Sugawara Yo, Arai Chika, Ouhara Kazuhisa, Nishi Hiromi, Mizuno Noriyoshi, Kawaguchi Hiroyuki, Shiba Hideki, Sugai Motoyuki, Komatsuzawa Hitoshi
Department of Biological Endodontics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.
Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.
Heliyon. 2024 Oct 28;10(21):e39827. doi: 10.1016/j.heliyon.2024.e39827. eCollection 2024 Nov 15.
The suppression of antimicrobial-resistant bacteria (ARB) is an important issue worldwide. In recent years, the presence of various ARB in the oral cavity has been reported, but the details remain unclear. Therefore, we aimed to isolate ARB from the oral cavity and investigate the factors affecting ARB colonization.
Third-generation cephalosporin- or carbapenem-resistant gram-negative bacteria (GN-ARB) were isolated from the oral and nasal cavities of 514 participants who visited the dental clinic, and the whole-genome sequences of all the isolates were obtained. Additionally, the tongue microbiota was analysed by 16S rRNA sequencing. The correlations of GN-ARB isolation with clinical status and the tongue microbiota were subsequently investigated.
Among 514 participants, 131 and 13 GN-ARB strains were isolated from the oral cavities of 93 participants (18.1 %) and from the nasal cavities of 12 participants (2.3 %). The ARB were mainly affiliated with spp. (39.7 %), spp. (14.5 %) and (18.3 %). We found a correlation between the isolation of oral GN-ARB and ageing/the number of teeth. There were no significant correlations between the presence of GN-ARB and tongue microbiota composition.
Our results suggest that the oral cavity is an important potential reservoir of GN-ARB and that ageing and tooth loss are risk factors for the presence of GN-ARB in the oral cavity.
抑制抗菌药物耐药菌（ARB）是全球范围内的一个重要问题。近年来，已有报道口腔中存在多种ARB，但具体情况仍不清楚。因此，我们旨在从口腔中分离ARB，并研究影响ARB定植的因素。
从514名到牙科诊所就诊的参与者的口腔和鼻腔中分离出对第三代头孢菌素或碳青霉烯耐药的革兰氏阴性菌（GN-ARB），并获得所有分离株的全基因组序列。此外，通过16S rRNA测序分析舌部微生物群。随后研究GN-ARB分离与临床状况和舌部微生物群的相关性。
在514名参与者中，分别从93名参与者（18.1%）的口腔和12名参与者（2.3%）的鼻腔中分离出131株和13株GN-ARB菌株。ARB主要隶属于 属（39.7%）、 属（14.5%）和 （18.3%）。我们发现口腔GN-ARB的分离与年龄增长/牙齿数量之间存在相关性。GN-ARB的存在与舌部微生物群组成之间没有显著相关性。
我们的结果表明，口腔是GN-ARB的一个重要潜在储存库，年龄增长和牙齿缺失是口腔中存在GN-ARB的危险因素。
