Duarte Catarina, Napoleão Patrícia, Freitas Teresa, Saldanha Carlota
Clin Hemorheol Microcirc. 2016;64(4):951-955. doi: 10.3233/CH-168023.
Acetylcholinesterase (AChE) is located on outer surface of erythrocyte membrane. Gender-related differences in erythrocyte AChE enzyme activity had been verified in young adults. It is also known that binding of acetylcholine (ACh) with AChE on erythrocyte membrane initiates a signal transduction mechanism that stimulates nitric oxide (NO) efflux.
This ex vivo study was done to compare the amount of NO efflux obtained from erythrocytes of healthy donors in males and females.
We included 66 gender age-matched healthy donors (40-60 years old). We performed quantification of erythrocyte NO efflux from erythrocytes and of the membrane AChE enzyme activity.
There are no significant differences in NO efflux from erythrocytes between men and women. Regarding AChE enzyme activity values, in this range of age, no differences between genders were obtained. However, the values of AChE enzyme activity in the third quartile of NO efflux values were significantly higher (p < 0.05) in women than in men.
The efflux of NO from erythrocyte of healthy humans did not change with gender. For the same range of values of NO efflux from erythrocytes, in both gender, it was verified higher values of AChE enzyme activity in women.
乙酰胆碱酯酶（AChE）位于红细胞膜的外表面。在年轻人中已证实红细胞AChE酶活性存在性别差异。还已知乙酰胆碱（ACh）与红细胞膜上的AChE结合会启动一种刺激一氧化氮（NO）外流的信号转导机制。
本体外研究旨在比较男性和女性健康供体红细胞中NO的外流量。
我们纳入了66名年龄匹配的健康供体（40 - 60岁）。我们对红细胞的NO外流量和膜AChE酶活性进行了定量分析。
男性和女性红细胞的NO外流量无显著差异。关于AChE酶活性值，在这个年龄范围内，未发现性别差异。然而，在NO外流量值的第三个四分位数中，女性的AChE酶活性值显著高于男性（p < 0.05）。
健康人红细胞中NO的外流不随性别而变化。对于相同范围的红细胞NO外流量值，在两个性别中，均证实女性的AChE酶活性值较高。
