脓毒性休克患者的可溶性CD40配体谱

Soluble CD40 ligand profiles in patients with septic shock.

作者信息

Napoleão Patrícia, Potapova Ekaterina, Moleirinho Sara, Saldanha Carlota, Messias António

机构信息

Carlota Saldanha Lab, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal.

Instituto de Bioquímica Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.

出版信息

Clin Hemorheol Microcirc. 2016;64(4):965-970. doi: 10.3233/CH-168026.

DOI:10.3233/CH-168026

PMID:27767979

Abstract

AIM

Soluble CD40 ligand (sCD40L) has been considered as a marker of thrombosis and inflammation in several diseases, including sepsis. Recent studies challenge this view and point to a role of sCD40L in vascular and endothelial function. An indication of that association in sepsis has not been obtained so far. Therefore, herein we evaluated association between sCD40L and markers of hemorheology and inflammation on context of septic shock.

METHODS

Time-changes of sCD40L levels over 72 hours of Intensive Care Unit (ICU) internment were assessed in 22 patients with septic shock and compared with 36 healthy volunteers. Association of sCD40L levels with erythrocyte deformability and aggregation (as markers of hemorheology), plasma concentrations of haemoglobin (Hb, as markers of endothelial function) and white blood cells (WBC) count (as marker of low-grade inflammation) were assessed in patients with septic shock.

RESULTS

At ICU admission, sCD40L concentrations in patients with septic shock were lower (p = 0.024) than levels of healthy volunteers. However, sCD40L did not change over 72 hours of internment (F = 2.1, p = 0.137). Soluble CD40L levels in patients with septic shock at ICU admission correlate with concentrations of Hb (r = 0.61, p = 0.00) and WBC count (r = 0.63, p = 0.00), but not to erythrocyte deformability (r≥0.157, p≤0.235) and aggregation (r≥-0.109, p≤0.192).

CONCLUSIONS

These results seem to highlight a possible association of sCD40L to endothelial function and inflammation in septic shock context.

摘要

目的

可溶性CD40配体（sCD40L）在包括脓毒症在内的多种疾病中被视为血栓形成和炎症的标志物。近期研究对这一观点提出质疑，并指出sCD40L在血管和内皮功能中发挥作用。目前尚未获得脓毒症中这种关联的证据。因此，我们在此评估脓毒性休克背景下sCD40L与血液流变学和炎症标志物之间的关联。

方法

评估22例脓毒性休克患者在重症监护病房（ICU）住院72小时内sCD40L水平的时间变化，并与36名健康志愿者进行比较。评估脓毒性休克患者中sCD40L水平与红细胞变形性和聚集性（作为血液流变学标志物）、血红蛋白（Hb，作为内皮功能标志物）血浆浓度以及白细胞（WBC）计数（作为低度炎症标志物）之间的关联。

结果

在ICU入院时，脓毒性休克患者的sCD40L浓度低于健康志愿者（p = 0.024）。然而，住院72小时内sCD40L没有变化（F = 2.1，p = 0.137）。ICU入院时脓毒性休克患者的可溶性CD40L水平与Hb浓度（r = 0.61，p = 0.00）和WBC计数（r = 0.63，p = 0.00）相关，但与红细胞变形性（r≥0.157，p≤0.235）和聚集性（r≥ - 0.109，p≤0.192）无关。