Yu Cuijuan, Wang Hai, Muscarella Aaron, Goldstein Amit, Zeng Huan-Chang, Bae Yangjin, Lee Brendan H I, Zhang Xiang H-F
Lester and Sue Smith Breast Center, Baylor College of Medicine; Department of Molecular and Cellular Biology, Baylor College of Medicine.
Graduate Program in Developmental Biology, Baylor College of Medicine.
J Vis Exp. 2016 Sep 26(115):53982. doi: 10.3791/53982.
Intra-iliac artery (IIA) injection is an efficient approach to introduce metastatic lesions of various cancer cells in animals. Compared to the widely used intra-cardiac and intra-tibial injections, IIA injection brings several advantages. First, it can deliver a large quantity of cancer cells specifically to hind limb bones, thereby providing spatiotemporally synchronized early-stage colonization events and allowing robust quantification and swift detection of disseminated tumor cells. Second, it injects cancer cells into the circulation without damaging the local tissues, thereby avoiding inflammatory and wound-healing processes that confound the bone colonization process. Third, IIA injection causes very little metastatic growth in non-bone organs, thereby preventing animals from succumbing to other vital metastases, and allowing continuous monitoring of indolent bone lesions. These advantages are especially useful for the inspection of progression from single cancer cells to multi-cell micrometastases, which has largely been elusive in the past. When combined with cutting-edge approaches of biological imaging and bone histology, IIA injection can be applied to various research purposes related to bone metastases.
髂内动脉（IIA）注射是在动物体内引入各种癌细胞转移灶的一种有效方法。与广泛使用的心脏内注射和胫骨内注射相比，IIA注射具有多个优点。首先，它可以将大量癌细胞特异性地输送到后肢骨骼，从而提供时空同步的早期定植事件，并允许对播散的肿瘤细胞进行可靠的定量和快速检测。其次，它将癌细胞注入循环系统而不损伤局部组织，从而避免了混淆骨定植过程的炎症和伤口愈合过程。第三，IIA注射在非骨器官中引起的转移性生长非常少，从而防止动物死于其他重要转移，并允许对惰性骨病变进行持续监测。这些优点对于检查从单个癌细胞到多细胞微转移的进展特别有用，而这在过去很大程度上一直难以实现。当与生物成像和骨组织学的前沿方法相结合时，IIA注射可应用于与骨转移相关的各种研究目的。
