Wada Yasushi, Higashide Tomomi, Nagata Atsushi, Sugiyama Kazuhisa
Ophthalmology and Visual Science, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan 2Ophthalmology, National Hospital Organization Kanazawa Medical Center, Kanazawa, Japan.
Ophthalmology and Visual Science, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5568-5575. doi: 10.1167/iovs.16-19945.
To investigate longitudinal changes in mean blur rate (MBR) measured by laser speckle flowgraphy (LSFG) in the rat optic nerve head (ONH), and the reproducibility of MBR.
Rats were dilated under general anesthesia. Intraocular pressure (IOP), blood pressure, ocular perfusion pressure (OPP), heart rate, and LSFG were measured 30 minutes later. Mean blur rate in the ONH was determined using LSFG-Micro and was subdivided into MBR of the total area (MA), vessel region (MV), and tissue region (MT). Mean blur rate measurements were repeated at 10, 11, 13, 19, and 20 weeks, then every 5 weeks until 60 weeks of age. Intrasession repeatability, intrasession reproducibility, and intersession reproducibility were evaluated.
Coefficient of variation of MBR was 0.3 to 6.2%, 1.3 to 5.2%, and 5.8 to 30.4% for intrasession repeatability, intrasession reproducibility, and intersession reproducibility, respectively. Mean blur rate of the total area, MV, and MT increased similarly until 19 weeks of age, but stabilized thereafter until 60 weeks. Mean blur rate of the total area in the inferior quadrant was significantly higher than in the temporal quadrant from 19 to 55 weeks. These changes exceeded a range of corresponding coefficient of reproducibility. There were no significant changes in IOP, blood pressure, or OPP during the experimental period.
Mean blur rate in the rat ONH changed over time, increased from 10 to 19 weeks of age, then stabilized until 60 weeks. Mean blur rate of the total area exhibited regional differences: higher in the inferior quadrant than in the temporal quadrant. Laser speckle flowgraphy-Micro may provide reliable information for evaluating longitudinal changes of rat ONH blood flow.
研究通过激光散斑血流图（LSFG）测量大鼠视神经乳头（ONH）平均模糊率（MBR）的纵向变化以及MBR的可重复性。
大鼠在全身麻醉下散瞳。30分钟后测量眼压（IOP）、血压、眼灌注压（OPP）、心率和LSFG。使用LSFG-Micro测定ONH的平均模糊率，并将其细分为总面积（MA）、血管区域（MV）和组织区域（MT）的MBR。在10、11、13、19和20周时重复测量平均模糊率，然后每5周测量一次，直至60周龄。评估测量期间内的重复性、测量期间内的可重复性和测量期间间的可重复性。
测量期间内的重复性、测量期间内的可重复性和测量期间间的可重复性的MBR变异系数分别为0.3%至6.2%、1.3%至5.2%和5.8%至30.4%。总面积、MV和MT的平均模糊率在19周龄前相似地增加，但此后稳定直至60周。在19至55周期间，下象限总面积的平均模糊率显著高于颞象限。这些变化超出了相应可重复性系数的范围。实验期间IOP、血压或OPP无显著变化。
大鼠ONH的平均模糊率随时间变化，在10至19周龄时增加，然后稳定直至60周。总面积的平均模糊率存在区域差异：下象限高于颞象限。激光散斑血流图-Micro可为评估大鼠ONH血流的纵向变化提供可靠信息。
