Fernandes Bruno, Matamá Teresa, Guimarães Diana, Gomes Andreia, Cavaco-Paulo Artur
CEB - Center of Biological Engineering, University of Minho, Braga, Portugal.
CBMA - Centre of Molecular and Environmental Biology, University of Minho, Braga, Portugal.
Pigment Cell Melanoma Res. 2016 Nov;29(6):707-712. doi: 10.1111/pcmr.12535. Epub 2016 Oct 22.
Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair.
据报道，黑色素定量是通过吸收光谱法进行的，通常在405纳米波长处。在此，我们建议采用荧光光谱法进行黑色素评估。在评估对外界刺激产生的黑色素生成的典型体外试验中，吸收光谱法明显高估了黑色素含量。该方法也无法区分非黑色素/无黑色素的对照细胞与实际能够进行黑色素生成的细胞。因此，荧光光谱法是黑色素定量的最佳方法，因为它被证明具有高度特异性和准确性，甚至能够检测到黑色素合成中的微小变化。该方法还可应用于斑马鱼胚胎和人类头发等更复杂生物基质中黑色素的定量。
