Wu Yang, Li Xueyong, Shi Xiaowen, Zhan Yingfei, Tu Hu, Du Yumin, Deng Hongbing, Jiang Linbin
School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, Guangxi University, Nanning 530004, China; Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Department of Plastic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Colloids Surf B Biointerfaces. 2017 Jan 1;149:271-279. doi: 10.1016/j.colsurfb.2016.10.030. Epub 2016 Oct 17.
When an efficient automated coating machine is used to process layer-by-layer (LBL) deposited nanofibrous mats, it causes an obvious planar effect on the surface of the mats, which can be eliminated through ultimate immersion. During this process, chitosan (CS) - rectorite (REC) intercalated composite films are built on the surface of cellulose acetate (CA) nanofibrous mats by a coating machine. Then, the immersion process is utilized to allow positively charged CS or CS-REC intercalated composites to uniformly assemble on the surface of negatively charged CA nanofibers. An investigation into the morphology of the resultant scaffolds confirms that the uniquely small pore size, high specific surface area and typically three-dimensional (3D) structure of nanofibrous mats remain present. The results of Fourier transform infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS) indicate that it is feasible to assemble nanofibrous mats using a coating machine. The intercalated structure of CS-REC is confirmed by the results of small-angle X-ray diffraction (SAXRD) and wide-angle X-ray diffraction (WAXRD). The results of the cell experiment and antibacterial test demonstrate that the addition of REC not only has little impact on the cytocompatibility of the mats but also enhances their ability to inhibit bacteria.
当使用高效的自动涂布机来处理逐层（LBL）沉积的纳米纤维垫时，会在垫的表面产生明显的平面效应，这可以通过最终浸泡来消除。在此过程中，通过涂布机在醋酸纤维素（CA）纳米纤维垫的表面构建壳聚糖（CS）-累托石（REC）插层复合膜。然后，利用浸泡过程使带正电的CS或CS-REC插层复合材料均匀地组装在带负电的CA纳米纤维表面。对所得支架的形态进行研究证实，纳米纤维垫独特的小孔径、高比表面积和典型的三维（3D）结构仍然存在。傅里叶变换红外光谱（FT-IR）和X射线光电子能谱（XPS）的结果表明，使用涂布机组装纳米纤维垫是可行的。小角X射线衍射（SAXRD）和广角X射线衍射（WAXRD）的结果证实了CS-REC的插层结构。细胞实验和抗菌测试的结果表明，REC的添加不仅对垫的细胞相容性影响很小，而且还增强了它们的抑菌能力。
