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通过层层沉积法制备壳聚糖/卵黄高磷蛋白抗菌膜。

Chitosan/phosvitin antibacterial films fabricated via layer-by-layer deposition.

机构信息

College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan, China.

College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan, China.

出版信息

Int J Biol Macromol. 2014 Mar;64:402-8. doi: 10.1016/j.ijbiomac.2013.12.016. Epub 2013 Dec 17.

DOI:10.1016/j.ijbiomac.2013.12.016
PMID:24360897
Abstract

Negatively charged phosvitin (PV) and positively charged chitosan (CS) were alternately deposited on negatively charged cellulose mats via layer-by-layer (LBL) self-assembly technique. The deposition of PV and CS was confirmed by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectra (FT-IR), and wide-angle X-ray diffraction (XRD). Morphologies of the LBL films coating mats were observed by scanning electron microscope (SEM). Thermal degradation properties were investigated by differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA). Additionally, the result of microbial inhibition assay indicated that the composite nanofibrous mats had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, which could be used for antimicrobial packing, tissue engineering, wound dressing, etc.

摘要

negatively charged phosvitin (PV) 和 positively charged chitosan (CS) 通过层层自组装技术交替沉积在带负电荷的纤维素垫上。通过 X 射线光电子能谱 (XPS)、傅里叶变换红外光谱 (FT-IR) 和广角 X 射线衍射 (XRD) 证实了 PV 和 CS 的沉积。通过扫描电子显微镜 (SEM) 观察 LBL 薄膜涂覆垫的形貌。通过差示扫描量热法 (DSC) 和热重分析 (TGA) 研究热降解性能。此外,微生物抑制试验的结果表明,复合纳米纤维垫对大肠杆菌和金黄色葡萄球菌具有优异的抗菌活性,可用于抗菌包装、组织工程、伤口敷料等。

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