Shi Rongfeng, Jin Yinpeng, Cao Chuanwu, Han Shilong, Shao Xiaowen, Meng Lingyu, Cheng Jie, Zhang Meiling, Zheng Jiayi, Xu Jun, Li Maoquan
Department of Interventional & Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, 301 Yanchang Road, Shanghai, 200072, People's Republic of China.
Shanghai Liver Diseases Research Center, The Nanjing Military Command, Shanghai, 200235, People's Republic of China.
Stem Cell Res Ther. 2016 Oct 22;7(1):155. doi: 10.1186/s13287-016-0412-2.
Diabetic foot ulcer (DFU) is an intractable diabetic complication. Patients suffering from diabetes mellitus (DM) frequently present with infected DFUs. In this study, a wound healing model on diabetic rat foot was established to mimic the pathophysiology of clinical patients who suffer from DFUs. Our study aimed to explore the localization of human adipose-derived stem cells (hADSCs) and the role of these cells in the repair of foot ulcerated tissue in diabetic rats, and thus to estimate the possibilities of adipose-derived stem cells for diabetic wound therapy.
Sprague-Dawley rats were used to establish diabetic models by streptozotocin injection. A full-thickness foot dorsal skin wound was created by a 5 mm skin biopsy punch and a Westcott scissor. These rats were randomly divided into two groups: the hADSC-treated group and the phosphate-buffered saline (PBS) control group. The hADSC or PBS treatment was delivered through the left femoral vein of rats. We evaluated the localization of hADSCs with fluorescence immunohistochemistry and the ulcer area and ulcerative histology were detected dynamically.
The hADSCs had a positive effect on the full-thickness foot dorsal skin wound in diabetic rats with a significantly reduced ulcer area at day 15. More granulation tissue formation, angiogenesis, cellular proliferation, and higher levels of growth factors expression were also detected in wound beds.
Our data suggest that hADSC transplantation has the potential to promote foot wound healing in diabetic rats, and transplantation of exogenous stem cells may be suitable for clinical application in the treatment of DFU.
糖尿病足溃疡（DFU）是一种难治性糖尿病并发症。糖尿病（DM）患者常出现感染性DFU。在本研究中，建立了糖尿病大鼠足部伤口愈合模型，以模拟临床DFU患者的病理生理学。本研究旨在探讨人脂肪源性干细胞（hADSCs）的定位及其在糖尿病大鼠足部溃疡组织修复中的作用，从而评估脂肪源性干细胞用于糖尿病伤口治疗的可能性。
采用链脲佐菌素注射法将Sprague-Dawley大鼠制成糖尿病模型。用5mm皮肤活检打孔器和韦斯科特剪刀造成足背部全层皮肤伤口。将这些大鼠随机分为两组：hADSC治疗组和磷酸盐缓冲盐水（PBS）对照组。通过大鼠左股静脉给予hADSC或PBS治疗。我们用荧光免疫组织化学法评估hADSCs的定位，并动态检测溃疡面积和溃疡组织学变化。
hADSCs对糖尿病大鼠足背部全层皮肤伤口有积极作用，在第15天时溃疡面积显著减小。在伤口床还检测到更多的肉芽组织形成、血管生成、细胞增殖以及更高水平的生长因子表达。
我们的数据表明，hADSC移植有可能促进糖尿病大鼠足部伤口愈合，外源性干细胞移植可能适用于DFU治疗的临床应用。
