Yoshikawa Chiaki, Qiu Jun, Shimizu Yoshihisa, Huang Chih-Feng, Gelling Onko-Jan, van den Bosch Edith
WPI Research Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki 305-0044, Japan.
DSM Ahead/TS, 6167 RD Geleen, Netherlands.
Mater Sci Eng C Mater Biol Appl. 2017 Jan 1;70(Pt 1):272-277. doi: 10.1016/j.msec.2016.08.066. Epub 2016 Aug 26.
Biofouling, an undesired adsorption of biological material to otherwise inert surfaces, is detrimental in medical, pharmaceutical, and other sectors. Concentrated polymer brushes (CPB) confer non-biofouling properties on modified surfaces but are cumbersome to fabricate. Here, a simple and versatile method of fabricating non-biofouling coatings for various substrates was developed using CPB-modified silica nanoparticles (SiPs). Concentrated poly(poly(ethylene glycol) methyl ether methacrylate) (PPEGMA) brushes were grafted on SiPs by surface-initiated atom transfer radical polymerization. CPB-SiPs were spin-coated onto silicon wafers or quartz crystal microbalance (QCM) sensor chips with phenyl azido cross-linkers. SiP cross-linking was then performed by ultra violet irradiation for 20s, or by heating at 120°C for 12h. Protein adsorption to coatings was studied by QCM approach and human umbilical vein endothelial cell adhesion to coatings was examined. SiP to cross-linker weight ratios were varied from 2.0/0.5 to 9.0/0.5 (wt/wt%) and the coatings almost completely suppressed protein adsorption and cell adhesion to treated surfaces. The coating was also applied to polymeric films, rendering these materials biofouling-resistant.
生物污垢是指生物材料意外吸附到原本惰性的表面上，这在医学、制药和其他领域都是有害的。浓缩聚合物刷（CPB）能赋予改性表面非生物污垢特性，但制备过程繁琐。在此，利用CPB改性的二氧化硅纳米颗粒（SiP）开发了一种简单且通用的方法，用于为各种基材制备非生物污垢涂层。通过表面引发的原子转移自由基聚合反应，在SiP上接枝了浓缩的聚（聚乙二醇甲基醚甲基丙烯酸酯）（PPEGMA）刷。将CPB-SiP与苯基叠氮交联剂旋涂到硅片或石英晶体微天平（QCM）传感器芯片上。然后通过紫外线照射20秒或在120°C加热12小时进行SiP交联。通过QCM方法研究蛋白质对涂层的吸附，并检测人脐静脉内皮细胞对涂层的粘附。SiP与交联剂的重量比在2.0/0.5至9.0/0.5（wt/wt%）之间变化，涂层几乎完全抑制了蛋白质吸附和细胞对处理表面的粘附。该涂层还应用于聚合物薄膜，使这些材料具有抗生物污垢性能。
