Jin Xingxing, Yuan Jiang, Shen Jian
Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
Colloids Surf B Biointerfaces. 2016 Sep 1;145:275-284. doi: 10.1016/j.colsurfb.2016.05.010. Epub 2016 May 9.
A low-fouling zwitterionic surface strategy has been proven to be promising and effective for repelling nonspecific adsorption of proteins, cells and bacteria, which may eventually induce adverse pathogenic problems such as thrombosis and infection. Herein, a multi-step process was developed by a combination of mussel-inspired chemistry and surface-initiated atom transfer radical polymerization (SI-ATRP) technique for improving hemocompatible and anti-biofouling properties. Polyethylene terephthalate (PET) sheets were first treated with dopamine, and then the bromoalkyl initiators were immobilized on the poly(dopamine) functionalized surfaces, followed by surface-initiated activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) of 2-(dimethylamino) ethyl methacrylate (DMAEMA) monomer. Subsequently, the resulting PET sheets were ring-opening reacted with 1,3-propiolactone (PL) and 1,3-propanesultone (PS) to afford polycarboxybetaine and polysulfobetaine brushes, respectively. Characterizations of the PET sheets were undertaken by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), atomic force microscope (AFM), water contact angle (WCA) measurements, and X-ray photoelectron spectroscopy (XPS) analysis, respectively. The conversion rates of PDMAEMA to polyzwitterions were evaluated by XPS analysis. The remained PDMAEMA(weak cationic) and formed zwitterions(neutral) would form a synergetic antifouling and antibacterial surface. Hemocompatible and anti-biofouling properties were evaluated by total adsorption of protein as well as the adhesion of platelet, cell and bacterium. Zwitterionic polymer brushes grafted PET sheets showed outstanding hemocompatibility featured on reduced platelet adhesion and repelled protein adsorption. Meanwhile, the grafted PET sheets exerted excellent anti-biofouling property characterized by the resisted adhesion of Escherichia coli and 3T3 cells. In summary, zwitterionic polymer brushed modified PET sheets have a great potential for biomedical applications.
一种低污染两性离子表面策略已被证明在排斥蛋白质、细胞和细菌的非特异性吸附方面很有前景且有效，而这些吸附最终可能引发诸如血栓形成和感染等不良致病问题。在此，通过将贻贝启发化学与表面引发原子转移自由基聚合（SI-ATRP）技术相结合，开发了一种多步工艺，以改善血液相容性和抗生物污染性能。首先用多巴胺处理聚对苯二甲酸乙二酯（PET）片材，然后将溴代烷基引发剂固定在聚多巴胺功能化表面上，接着通过甲基丙烯酸2-（二甲氨基）乙酯（DMAEMA）单体的电子转移活化剂再生原子转移自由基聚合（ARGET ATRP）进行表面引发聚合。随后，所得的PET片材分别与1,3-丙内酯（PL）和1,3-丙烷磺内酯（PS）进行开环反应，分别得到聚羧基甜菜碱和聚磺基甜菜碱刷。分别通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、原子力显微镜（AFM）、水接触角（WCA）测量和X射线光电子能谱（XPS）分析对PET片材进行表征。通过XPS分析评估PDMAEMA向聚两性离子的转化率。残留的PDMAEMA（弱阳离子）和形成的两性离子（中性）将形成协同的防污和抗菌表面。通过蛋白质的总吸附以及血小板、细胞和细菌的粘附来评估血液相容性和抗生物污染性能。接枝有两性离子聚合物刷的PET片材表现出优异的血液相容性，其特征在于血小板粘附减少和蛋白质吸附被排斥。同时，接枝的PET片材表现出优异的抗生物污染性能，其特征在于对大肠杆菌和3T3细胞的粘附具有抗性。总之，两性离子聚合物刷修饰的PET片材在生物医学应用方面具有巨大潜力。
