Kojima T, Yashiki M, Miyazaki T, Chikasue F, Ohtani M
Department of Legal Medicine, Hiroshima University School of Medicine, Japan.
Forensic Sci Int. 1989 Jun;41(3):245-53. doi: 10.1016/0379-0738(89)90217-x.
A 23-year-old male attempted suicide by ingesting approximately 50 ml of 5% fenitrothion emulsion, and vomited soon afterwards. He was admitted to a hospital about 3 h after ingestion. He recovered and was discharged from hospital 3 days after admission. The serum cholinesterase activity (normal range: 175-440 I.U.) was only 29 at 3 h, 32 at 1 day, 59 at 2 days and 75 at 3 days after ingestion. Fenitrothion and its metabolites in the body fluids were extracted by an Extrelut column extraction method, detected by a gas chromatograph equipped with either a hydrogen flame ionization detector or a flame photometric detector, and confirmed by a gas chromatograph-mass spectrometer. Fenitrothion concentration in the blood was 169.5 ng/g at 3 h after ingestion. The half life of blood fenitrothion concentration was found to be about 4.5 h. Fenitrothion metabolites, 3-methyl-4-nitrophenol, aminofenitrothion, aminofenitroxon, acetylaminofenitroxon and S-methylfenitrothion, were detected in the urine samples. All of them except S-methylfenitrothion were detected in the urine samples collected up to 62 h after ingestion. It would appear therefore that fenitrothion poisoning can be determined by detection and analysis of the metabolites in urine even if fenitrothion has not been detected in the blood.
一名23岁男性摄入约50毫升5%杀螟硫磷乳剂企图自杀，随后不久呕吐。摄入后约3小时他被送往医院。他康复后于入院3天后出院。摄入后3小时血清胆碱酯酶活性（正常范围：175 - 440国际单位）仅为29，1天时为32，2天时为59，3天时为75。通过埃特拉路特柱萃取法提取体液中的杀螟硫磷及其代谢物，用配备氢火焰离子化检测器或火焰光度检测器的气相色谱仪进行检测，并用气相色谱 - 质谱仪进行确证。摄入后3小时血液中杀螟硫磷浓度为169.5纳克/克。发现血液中杀螟硫磷浓度的半衰期约为4.5小时。在尿液样本中检测到杀螟硫磷的代谢物3 - 甲基 - 4 - 硝基苯酚、氨基杀螟硫磷、氨基杀螟氧磷、乙酰氨基杀螟氧磷和S - 甲基杀螟硫磷。除S - 甲基杀螟硫磷外，所有这些代谢物在摄入后长达62小时收集的尿液样本中均被检测到。因此，即使血液中未检测到杀螟硫磷，似乎也可以通过检测和分析尿液中的代谢物来确定杀螟硫磷中毒。
