乙烯促进灵芝菌丝体生长和灵芝酸生物合成。
Ethylene promotes mycelial growth and ganoderic acid biosynthesis in Ganoderma lucidum.
作者信息
Zhang Guang, Ren Ang, Wu Fengli, Yu Hanshou, Shi Liang, Zhao Mingwen
机构信息
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, No.1 Weigang, Nanjing, 210095, Jiangsu, People's Republic of China.
出版信息
Biotechnol Lett. 2017 Feb;39(2):269-275. doi: 10.1007/s10529-016-2238-5. Epub 2016 Oct 22.
OBJECTIVE
To investigate the effects of ethylene, in the form of ethephon (2-chloroethylphosphonic acid), on mycelial growth and ganoderic acid (GA) accumulation in the higher basidiomycete Ganoderma lucidum.
RESULTS
Treatment with both 10 and 15 mM ethephon enhanced the growth of G. lucidum on solid CYM plates and in CYM liquid medium. After optimization using response surface methodology, GA reached 33 mg/g dry cell weight (DW), an increase of 90 %, compared with the control. Lanosterol and squalene contents were 31 and 2.4 μg/g DW, being increased by 1.2- and 0.6-fold, respectively, in response to ethephon. Additionally, the transcriptional levels of hydroxymethylglutaryl-CoA reductase, squalene synthase and oxidosqualene cyclase were up-regulated by 2.6-, 4.3- and 3.8-fold, respectively, compared with the control group.
CONCLUSIONS
This approach provides an efficient strategy for improving GA accumulation in G. lucidum, with potential future applications.
目的
研究以乙烯利（2-氯乙基膦酸）形式存在的乙烯对高等担子菌灵芝菌丝体生长和灵芝酸（GA）积累的影响。
结果
10 mM和15 mM乙烯利处理均促进了灵芝在固体CYM平板和CYM液体培养基中的生长。采用响应面法优化后，GA含量达到33 mg/g干细胞重量（DW），与对照相比增加了90%。羊毛甾醇和角鲨烯含量分别为31 μg/g DW和2.4 μg/g DW，乙烯利处理后分别增加了1.2倍和0.6倍。此外，与对照组相比，羟甲基戊二酰辅酶A还原酶、角鲨烯合酶和氧化角鲨烯环化酶的转录水平分别上调了2.6倍、4.3倍和3.8倍。
结论
该方法为提高灵芝中GA的积累提供了一种有效的策略，具有潜在的未来应用前景。
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