Hu Yanru, Chai Qianqian, Wang Yue, Chen Yujie, Dong Haozhe, Shen Jinwen, Qi Yuancheng, Yu Haiyou, Wang Fengqin, Wen Qing
Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.
College of Food and Bioengineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450002, China.
Life (Basel). 2022 Jun 17;12(6):915. doi: 10.3390/life12060915.
(Jacq.) P. Kumm has high medicinal value, but few studies exist on regulating secondary metabolite biosynthesis. Environmental factors play a substantial role in the accumulation of microbial secondary metabolites. In this study, the effects of heat stress (24 h) and salicylic acid (0.05 mmol/L) treatment on the secondary metabolism of were analyzed by metabolome, transcriptome, and gene differential expression analysis. Metabolome and transcriptome analyses showed that salicylic acid significantly increased the accumulation of antibiotics and polyketones, while heat stress increased the accumulation of flavonoids, polyketones, terpenoids, and polysaccharides. The content and the biosynthetic genes expression of heparin were markedly increased by heat stress, and the former was increased by 4565.54-fold. This study provides a reference for future studies on secondary metabolite accumulation in edible fungi.
（雅克）P. 库姆具有很高的药用价值，但关于调节其次级代谢产物生物合成的研究很少。环境因素在微生物次级代谢产物的积累中起着重要作用。在本研究中，通过代谢组学、转录组学和基因差异表达分析，分析了热应激（24小时）和水杨酸（0.05毫摩尔/升）处理对（此处原文缺失具体研究对象）次级代谢的影响。代谢组学和转录组学分析表明，水杨酸显著增加了抗生素和聚酮类化合物的积累，而热应激增加了黄酮类化合物、聚酮类化合物、萜类化合物和多糖的积累。热应激显著增加了肝素的含量及其生物合成基因的表达，前者增加了4565.54倍。本研究为今后食用菌次级代谢产物积累的研究提供了参考。
