Kaur Khushwinder, Kumar Raj, Goel Sumit, Uppal Shivani, Bhatia Alka, Mehta S K
Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India.
Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India.
Ultrason Sonochem. 2017 Jan;34:173-182. doi: 10.1016/j.ultsonch.2016.05.037. Epub 2016 May 21.
The main aim of the present work was to prepare TPGS stabilized D-α-Tocopherol, lemon oil, tween-80, and water nanoemulsion by low cost and highly effective sonication method. The prepared nanoemulsion showed good stability for 60days at variable temperature conditions i.e. 4, 25 and 37°C. The tolerance of the prepared nanoemulsion to salt (50mM-500mM) and pH (pH 2-pH 7.4) was also studied. The morphology and droplet size of pure and quinine loaded nanoemulsion was characterized with transmission electron microscopy. The prepared formulation was transparent and the obtained average particle size ranged between 25nm and 35nm. The nanoemulsion was found to be non toxic. The cell viability study of pure nanoemulsion carried out on Hep G2 cells revealed that the cell viability was 100%. The formulation further exhibited high quinine loading and release capacity with cumulative release up to 76±2% and 65±2% at pH 7.4 and pH 5.5 respectively. The interaction between quinine and vitamins (riboflavin, thiamine and biotin) was also carried out (aqueous medium). The study revealed that riboflavin had strong interaction with quinine and vitamins vis-à-vis thiamine and biotin.
本研究的主要目的是通过低成本且高效的超声处理方法制备TPGS稳定的D-α-生育酚、柠檬油、吐温80和水纳米乳液。所制备的纳米乳液在4、25和37°C等可变温度条件下60天内表现出良好的稳定性。还研究了所制备的纳米乳液对盐（50mM - 500mM）和pH（pH 2 - pH 7.4）的耐受性。用透射电子显微镜对纯的和负载奎宁的纳米乳液的形态和液滴尺寸进行了表征。所制备的制剂是透明的，获得的平均粒径在25nm至35nm之间。发现该纳米乳液无毒。对Hep G2细胞进行的纯纳米乳液的细胞活力研究表明细胞活力为100%。该制剂在pH 7.4和pH 5.5时分别进一步表现出高奎宁负载和释放能力，累积释放率高达76±2%和65±2%。还进行了奎宁与维生素（核黄素、硫胺素和生物素）之间的相互作用研究（在水介质中）。研究表明，相对于硫胺素和生物素，核黄素与奎宁和维生素有很强的相互作用。
