Chau I Y, Dagani D, Archer M C
J Natl Cancer Inst. 1978 Aug;61(2):517-21.
The oxidative dealkylation of dimethylnitrosamine (DMN), diethylnitrosamine (DEN), and methylethylnitrosamine (MEN) by Sprague-Dawley rat liver microsomes was studied by a colorimetric assay for the simultaneous analysis of formaldehyde and acetaldehyde. Dealkylation in each instance followed Michaelis-Menten kinetics, but the Michaelis constant (Km) for DEN was an order of magnitude smaller than the Km for DMN. Km values for demethylation and deethylation of MEN were intermediate between those for DMN and DEN. DMN inhibited-deethylase, and DEN inhibited DMN-demethylase activity. The inhibition was of a mixed type in both instances.
通过比色法同时分析甲醛和乙醛，研究了Sprague-Dawley大鼠肝微粒体对二甲基亚硝胺（DMN）、二乙基亚硝胺（DEN）和甲基乙基亚硝胺（MEN）的氧化脱烷基作用。在每种情况下，脱烷基作用均遵循米氏动力学，但DEN的米氏常数（Km）比DMN的Km小一个数量级。MEN的去甲基化和去乙基化的Km值介于DMN和DEN之间。DMN抑制去乙基酶，DEN抑制DMN-去甲基酶活性。在这两种情况下，抑制作用均为混合型。
