• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

沙眼衣原体中脂多糖的免疫电子显微镜观察

Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis.

作者信息

Birkelund S, Lundemose A G, Christiansen G

机构信息

Institute of Medical Microbiology, University of Aarhus, Denmark.

出版信息

Infect Immun. 1989 Oct;57(10):3250-3. doi: 10.1128/iai.57.10.3250-3253.1989.

DOI:10.1128/iai.57.10.3250-3253.1989
PMID:2777384
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260801/
Abstract

Monoclonal antibodies (MAb) specific for Chlamydia trachomatis lipopolysaccharide (LPS) and major outer membrane protein (MOMP) were used for immunoelectron microscopy analysis. MAb specific for MOMP showed strong reaction with the chlamydial surface, whereas MAb specific for LPS showed strong association of gold particles with the periphery of the chlamydial body. After fixation of the chlamydia cells, the reactivity was, however, similar to the anti-MOMP reactivity. Enzyme-linked immunosorbent assay showed that MAb specific for LPS could remove LPS from the chlamydial membrane.

摘要

针对沙眼衣原体脂多糖（LPS）和主要外膜蛋白（MOMP）的单克隆抗体（MAb）用于免疫电子显微镜分析。针对MOMP的MAb与衣原体表面有强烈反应，而针对LPS的MAb显示金颗粒与衣原体原体周边有强烈结合。然而，在衣原体细胞固定后，其反应性与抗MOMP反应性相似。酶联免疫吸附测定表明，针对LPS的MAb可从衣原体膜上去除LPS。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d95a/260801/79eb6ddaecc7/iai00070-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d95a/260801/79eb6ddaecc7/iai00070-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d95a/260801/79eb6ddaecc7/iai00070-0336-a.jpg

相似文献

1
Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis.沙眼衣原体中脂多糖的免疫电子显微镜观察
Infect Immun. 1989 Oct;57(10):3250-3. doi: 10.1128/iai.57.10.3250-3253.1989.
2
Lipopolysaccharide in cells infected by Chlamydia trachomatis.沙眼衣原体感染细胞中的脂多糖。
Microbiology (Reading). 1994 Aug;140 ( Pt 8):1995-2002. doi: 10.1099/13500872-140-8-1995.
3
Lack of neutralization of Chlamydia trachomatis infection by high avidity monoclonal antibodies to surface-exposed major outer membrane protein variable domain IV.高亲和力单克隆抗体不能中和沙眼衣原体感染表面暴露的主要外膜蛋白可变区 IV。
Mol Immunol. 2023 Nov;163:163-173. doi: 10.1016/j.molimm.2023.09.015. Epub 2023 Oct 4.
4
Chemical cross-linking of Chlamydia trachomatis.
Infect Immun. 1988 Mar;56(3):654-9. doi: 10.1128/iai.56.3.654-659.1988.
5
Alteration of Chlamydia trachomatis biologic behavior in synovial membranes. Suppression of surface antigen production in reactive arthritis and Reiter's syndrome.沙眼衣原体在滑膜中的生物学行为改变。反应性关节炎和赖特综合征中表面抗原产生的抑制。
Arthritis Rheum. 1995 Oct;38(10):1410-7. doi: 10.1002/art.1780381008.
6
Overexpression and surface localization of the Chlamydia trachomatis major outer membrane protein in Escherichia coli.沙眼衣原体主要外膜蛋白在大肠杆菌中的过表达及表面定位
Mol Microbiol. 1992 May;6(9):1087-94. doi: 10.1111/j.1365-2958.1992.tb01545.x.
7
Intracellular persistence of chlamydial major outer-membrane protein, lipopolysaccharide and ribosomal RNA after non-productive infection of human monocytes with Chlamydia trachomatis serovar K.沙眼衣原体血清型K对人单核细胞进行非生产性感染后衣原体主要外膜蛋白、脂多糖和核糖体RNA的细胞内持续存在情况
J Med Microbiol. 1993 Apr;38(4):278-85. doi: 10.1099/00222615-38-4-278.
8
Staining of surface antigens of Chlamydia trachomatis L2 in tissue culture.
Infect Immun. 1992 Oct;60(10):4433-8. doi: 10.1128/iai.60.10.4433-4438.1992.
9
The role of lipopolysaccharide in the exposure of protective antigenic sites on the major outer membrane protein of Chlamydia trachomatis.脂多糖在沙眼衣原体主要外膜蛋白上保护性抗原位点暴露中的作用。
J Gen Microbiol. 1992 Jun;138(6):1221-7. doi: 10.1099/00221287-138-6-1221.
10
Mimicry of a neutralizing epitope of the major outer membrane protein of Chlamydia trachomatis by anti-idiotypic antibodies.抗独特型抗体对沙眼衣原体主要外膜蛋白中和表位的模拟
Infect Immun. 1994 Feb;62(2):341-7. doi: 10.1128/iai.62.2.341-347.1994.

引用本文的文献

1
Induction of interferon-stimulated genes by Chlamydia pneumoniae in fibroblasts is mediated by intracellular nucleotide-sensing receptors.肺炎衣原体在成纤维细胞中诱导干扰素刺激基因是通过细胞内核苷酸感应受体介导的。
PLoS One. 2010 Apr 6;5(4):e10005. doi: 10.1371/journal.pone.0010005.
2
Diagnosis and assessment of trachoma.沙眼的诊断与评估
Clin Microbiol Rev. 2004 Oct;17(4):982-1011, table of contents. doi: 10.1128/CMR.17.4.982-1011.2004.
3
Chlamydia and sudden infant death syndrome. A study of 166 SIDS and 30 control cases.

本文引用的文献

1
Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization.抗沙眼衣原体单克隆抗体：抗体特异性及抗原特性
J Immunol. 1982 Mar;128(3):1083-9.
2
Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes.利用抗体-金珠免疫探针进行特定淋球菌大分子的超微结构定位
Infect Immun. 1984 Nov;46(2):361-6. doi: 10.1128/iai.46.2.361-366.1984.
3
Human serology in Chlamydia trachomatis infection with microimmunofluorescence.沙眼衣原体感染的微量免疫荧光法人体血清学研究
Int J Legal Med. 1990 Dec;104(1):3-7. doi: 10.1007/BF01816475.
4
Topological analysis of Chlamydia trachomatis L2 outer membrane protein 2.沙眼衣原体L2外膜蛋白2的拓扑分析
J Bacteriol. 1998 Nov;180(21):5784-7. doi: 10.1128/JB.180.21.5784-5787.1998.
5
Induction of alpha/beta interferon and dependent nitric oxide synthesis during Chlamydia trachomatis infection of McCoy cells in the absence of exogenous cytokine.在无外源性细胞因子情况下，沙眼衣原体感染 McCoy 细胞期间α/β干扰素的诱导及相关一氧化氮合成
Infect Immun. 1996 Oct;64(10):3951-6. doi: 10.1128/iai.64.10.3951-3956.1996.
6
Establishment of a particle-counting method for purified elementary bodies of chlamydiae and evaluation of sensitivities of the IDEIA Chlamydia kit and DNA probe by using the purified elementary bodies.衣原体纯化原体颗粒计数方法的建立及运用该纯化原体对衣原体免疫诊断酶免疫分析试剂盒和DNA探针敏感性的评估。
J Clin Microbiol. 1992 Nov;30(11):2911-6. doi: 10.1128/jcm.30.11.2911-2916.1992.
7
Staining of surface antigens of Chlamydia trachomatis L2 in tissue culture.
Infect Immun. 1992 Oct;60(10):4433-8. doi: 10.1128/iai.60.10.4433-4438.1992.
J Infect Dis. 1974 Oct;130(4):388-97. doi: 10.1093/infdis/130.4.388.
4
Effect of prior sexually transmitted disease on the isolation of Chlamydia trachomatis.既往性传播疾病对沙眼衣原体分离的影响。
Sex Transm Dis. 1987 Jul-Sep;14(3):160-4. doi: 10.1097/00007435-198707000-00008.
5
Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis.保护性单克隆抗体识别位于沙眼衣原体主要外膜蛋白上的表位。
J Immunol. 1987 Jan 15;138(2):575-81.
6
Tear and serum antibody response to Chlamydia trachomatis antigens during acute chlamydial conjunctivitis in monkeys as determined by immunoblotting.通过免疫印迹法测定猴子急性衣原体性结膜炎期间对沙眼衣原体抗原的泪液和血清抗体反应。
Infect Immun. 1987 Jan;55(1):93-8. doi: 10.1128/iai.55.1.93-98.1987.
7
Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry.蛋白质稳定的金溶胶的非特异性结合作为免疫细胞化学中误差的一个来源。
Eur J Cell Biol. 1986 Aug;41(2):326-38.
8
Steric hindrance of antibody binding to surface proteins of Coxiella burnetti by phase I lipopolysaccharide.I相脂多糖对抗体结合伯纳特立克次体表面蛋白的空间位阻作用。
Infect Immun. 1988 Apr;56(4):802-7. doi: 10.1128/iai.56.4.802-807.1988.
9
The function of antibody and complement in the lysis of bacteria.抗体和补体在细菌溶解中的作用。
Rev Infect Dis. 1987 Sep-Oct;9 Suppl 5:S537-45. doi: 10.1093/clinids/9.supplement_5.s537.
10
Attempted oral immunization with chlamydial lipopolysaccharide subunit vaccine.尝试用衣原体脂多糖亚单位疫苗进行口服免疫。
Invest Ophthalmol Vis Sci. 1987 Oct;28(10):1722-6.