Taylor H R, Prendergast R A
Wilmer Institute, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205.
Invest Ophthalmol Vis Sci. 1987 Oct;28(10):1722-6.
The effects of oral immunization with a recombinant vaccine expressing chlamydial lipopolysaccharide (LPS) on subsequent ocular challenge with Chlamydia trachomatis were studied in cynomolgus monkeys. Groups of four or five monkeys were given an oral vaccine containing 5 X 10(8) parent or recombinant Escherichia coli on days 0, 14, and 35 and were challenged with either 2 X 10(3) or 5 X 10(3) inclusion forming units of viable purified elementary bodies on day 42. On clinical and microbiologic grounds, oral immunization failed to protect monkeys against subsequent ocular challenge. Antichlamydial IgG or IgA antibodies were not induced by oral vaccination, and the antibody response following ocular challenge was similar in vaccinated and nonvaccinated animals. Paradoxically, however, while nonvaccinated control animals developed antibodies against chlamydial LPS detectable by immunoblotting after chlamydial challenge, the LPS vaccinated animals did not. This study demonstrates that the oral recombinant vaccine expressing chlamydial LPS was ineffective in protecting against chlamydial eye infection and strongly suggests that chlamydial LPS may not be an important antigen for protective immunity against chlamydia.
在食蟹猴中研究了用表达衣原体脂多糖（LPS）的重组疫苗进行口服免疫对随后沙眼衣原体眼部攻击的影响。将四或五只猴子分为一组，在第0、14和35天给予含有5×10⁸亲本或重组大肠杆菌的口服疫苗，并在第42天用2×10³或5×10³个活的纯化原体的包涵体形成单位进行攻击。基于临床和微生物学依据，口服免疫未能保护猴子免受随后的眼部攻击。口服疫苗未诱导抗衣原体IgG或IgA抗体，并且在接种疫苗和未接种疫苗的动物中，眼部攻击后的抗体反应相似。然而，矛盾的是，虽然未接种疫苗的对照动物在衣原体攻击后通过免疫印迹可检测到抗衣原体LPS抗体，但接种LPS疫苗的动物却没有。这项研究表明，表达衣原体LPS的口服重组疫苗在预防衣原体眼部感染方面无效，并强烈提示衣原体LPS可能不是抗衣原体保护性免疫的重要抗原。
