Koeppen K M, Boll I T
Klin Wochenschr. 1978 Aug 1;56(15):779-80. doi: 10.1007/BF01476767.
Blood cells from 68 patients with untreated acute myeloid luekemia were cultured in RPMI-medium without stimulating factors up to ten days. The cultures showed in part maturation and proliferation to monocytes-macrophages, in part to promyelocytes, myelocytes and Pelger-like cells, in part we did not find any differentiation or the cultures were degenerated during the first days. Retrospectively we found that in the 16 blood cell cultures with capacity to differentiation into the monocyte-macrophages-system 5 patients had a smouldering leukemia. Our preliminary evidences suggest that the diagnosis "smouldering leukemia" is to be found with out in vitro culture system. Further analysis suggest that patients with acute leukemia whose blood cells have the capacity for maturation to monocytes-macrophages or to promyelocytes, myelocytes and Pelger-like cells have a better chance of achieving a complete remission and a longer median survival time.
将68例未经治疗的急性髓系白血病患者的血细胞在无刺激因子的RPMI培养基中培养长达10天。培养结果显示，部分细胞成熟并增殖为单核细胞-巨噬细胞，部分增殖为早幼粒细胞、中幼粒细胞和Pelger样细胞，部分在最初几天未发现任何分化或培养物退化。回顾性分析发现，在16例有能力分化为单核细胞-巨噬细胞系统的血细胞培养物中，有5例患者患有冒烟型白血病。我们的初步证据表明，“冒烟型白血病”的诊断可以通过体外培养系统得出。进一步分析表明，急性白血病患者若其血细胞有能力成熟为单核细胞-巨噬细胞或早幼粒细胞、中幼粒细胞和Pelger样细胞，则更有可能实现完全缓解，且中位生存时间更长。
