Massazza G, Tomasoni A, Lucchini V, Allavena P, Erba E, Colombo N, Mantovani A, D'Incalci M, Mangioni C, Giavazzi R
Mario Negri Institute for Pharmacological Research, Bergamo.
Int J Cancer. 1989 Sep 15;44(3):494-500. doi: 10.1002/ijc.2910440320.
Human ovarian carcinomas (HOC) were established s.c. and i.p. in nude mice and the biological characteristics were investigated for 4 xenografts. HOC8 and HOC18, derived respectively from a primary tumor of the ovary and a pleural effusion (from 2 different patients) were established s.c. in nude mice. HOC10 and HOC22, derived from the ascites of 2 patients, were directly established as ascites after i.p. injection in nude mice. The s.c. and i.p. growth behavior of the 4 HOC lines was investigated. HOC18, HOC8 and HOC22 cells produced progressively growing tumor after s.c. injection but HOC10 ascites would not grow s.c. The cell suspension derived from HOC18 only produced carcinomatosis upon i.p. injection, while HOC8 cells produced both ascites and carcinomatosis. The 2 ascites HOC10 and HOC22 produced ascites in nude mice, but only HOC22 formed i.p. carcinomatosis. Histopathological characteristics of the patients' primary tumors persisted in nude mice, regardless of the site of tumor implantation. DNA histograms of the xenografts closely matched the patients' tumors and remained stable at different passages. Cisplatin, adriamycin and cyclophosphamide given i.v. were tested against HOC8 and HOC18 growing s.c. and HOC22 and HOC10 growing i.p. HOC8 showed a significant response to DDP and almost no sensitivity to ADR and CTX. HOC18 showed only moderate growth delay with all 3 drugs. Mice bearing HOC10 and HOC22 ascites had a prolonged survival time after DDP and ADR treatment.
人卵巢癌（HOC）在裸鼠体内皮下和腹腔接种建立模型，并对4种异种移植瘤的生物学特性进行了研究。分别源自卵巢原发性肿瘤和胸腔积液（来自2名不同患者）的HOC8和HOC18在裸鼠体内皮下接种建立模型。源自2名患者腹水的HOC10和HOC22在裸鼠腹腔注射后直接建立腹水模型。研究了4种HOC细胞系的皮下和腹腔生长行为。皮下注射后，HOC18、HOC8和HOC22细胞产生逐渐生长的肿瘤，但HOC10腹水不能在皮下生长。源自HOC18的细胞悬液腹腔注射后仅产生癌转移，而HOC8细胞则产生腹水和癌转移。两种腹水型HOC10和HOC22在裸鼠体内产生腹水，但只有HOC22形成腹腔癌转移。患者原发性肿瘤的组织病理学特征在裸鼠体内持续存在，与肿瘤植入部位无关。异种移植瘤的DNA直方图与患者肿瘤密切匹配，且在不同传代时保持稳定。对皮下生长的HOC8和HOC18以及腹腔生长的HOC22和HOC10进行了静脉注射顺铂、阿霉素和环磷酰胺的测试。HOC8对顺铂有显著反应，对阿霉素和环磷酰胺几乎不敏感。HOC18对所有3种药物仅表现出中度生长延迟。携带HOC10和HOC22腹水的小鼠在顺铂和阿霉素治疗后存活时间延长。
