McGillivery D J, Yong W K, Riffkin G G, Adler B
Int J Parasitol. 1989 Aug;19(5):473-8. doi: 10.1016/0020-7519(89)90076-3.
Under similar extraction conditions, Triton X-100 sonicates gave higher yields of protein from third stage larvae and adult O. circumcincta than seven other detergents tested. Using sera from sheep which had been experimentally defined by both immunological and parasitological parameters as being either resistant or susceptible to O. circumcincta, a molecule from Triton X-100 extracts of third stage O. circumcincta larvae was identified which reacted preferentially with sera from resistant sheep. This molecule has a molecular weight of 31,000 and preliminary characterization studies revealed it to be a glycoprotein which was not found in later larval stages or adult worms. Antibodies to this 31,000 mol.wt antigen were present in sera of sheep as early as 3 weeks after experimental infection with O. circumcincta.
在相似的提取条件下，与其他七种受试去污剂相比，Triton X-100超声处理从环纹奥斯特线虫第三期幼虫和成虫中获得的蛋白质产量更高。使用通过免疫和寄生虫学参数在实验上被定义为对环纹奥斯特线虫有抗性或易感性的绵羊血清，鉴定出一种来自环纹奥斯特线虫第三期幼虫的Triton X-100提取物中的分子，该分子优先与抗性绵羊的血清发生反应。该分子的分子量为31,000，初步表征研究表明它是一种糖蛋白，在后期幼虫阶段或成虫中未发现。早在用环纹奥斯特线虫进行实验感染后3周，绵羊血清中就存在针对这种31,000分子量抗原的抗体。
